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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
other: hydrolysis product
Adequacy of study:
supporting study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
review article or handbook
Title:
Unnamed
Year:
2003

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Imidazole
EC Number:
206-019-2
EC Name:
Imidazole
Cas Number:
288-32-4
Molecular formula:
C3H4N2
IUPAC Name:
1H-imidazole
Details on test material:
99,76%

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage

Study design

Total exposure duration:
30 min

Results and discussion

Effect concentrations
Duration:
30 min
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Details on results:
Endpoint: growth rate
Exposure period: 72 hour(s)
Unit: mg/l Analytical monitoring: no
NOEC: = 25
LOEC: = 50
EC10: = 63.7
EC50: = 133

Any other information on results incl. tables

- The effects are related to the nominal concentrations effect values based on biomass:

EbC10 (72 h) = 58.7 mg/L

EbC10 (96 h) = 33.6 mg/L

EbC50 (72 h) = 126.8 mg/L

EbC50 (96 h) = 81.8 mg/L

NOEC (72 h) = 10.0 mg/L

LOEC (72 h) = 25.0 mg/L

effect values based on growth rate:

EuC10 (96 h) = 20.0 mg/L

EuC50 (96 h) = 129.6 mg/L

- pH values at test start (w/o algae) and after 96 h (inoculated assays):

concentration (mg/L) pH (0 h) pH (96 h)

control         7.84     7.92

5             7.87     7.97

10             7.86     7.98

25             7.87     7.99

50             7.91     8.01

100             8.00     8.05

250             8.17     8.24

Applicant's summary and conclusion