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EC number: 200-431-6 | CAS number: 59-50-7
Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Description of key information
The substance chlorocresol is not genotoxic
Link to relevant study records
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Qualifier:
- according to guideline
- Guideline:
- other:
- Principles of method if other than guideline:
- Publication
- GLP compliance:
- not specified
- Type of assay:
- other: Salmonella/mammalian microsome test
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S 9 microsome fraction from Aroclor 1254 induced male Wistar rat liver
- Test concentrations with justification for top dose:
- 1.28, 6.4, 32, 160, or 800 ug
- Vehicle / solvent:
- DMSO
- Untreated negative controls:
- yes
- Remarks:
- DMSO
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: Sodium azide
- Remarks:
- strains TA 1535 and TA 100 without S9
- Untreated negative controls:
- yes
- Remarks:
- DMSO
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- strains TA 1537 and TA 98 without S9
- Untreated negative controls:
- yes
- Remarks:
- DMSO
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: 2-antramine
- Remarks:
- For all strains with S9
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'. Remarks: no data
- Conclusions:
- Interpretation of results (migrated information):
negative
4-chloro-3-methylphenol at doses 1.28, 6.4, 32, 160 and 800 ug per plate did not show mutagenic potential in Salmonella tester strain TA 1585, TA 100, TA 1537, and TA 98 with and without S9. - Executive summary:
4-chloro-3-methylphenol at doses 1.28, 6.4, 32, 160 and 800 ug per plate did not show mutagenic potential in Salmonella tester strain TA 1585, TA 100, TA 1537, and TA 98 with and without S9.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Additional information
Additional information from genetic toxicity in vitro:
Based on studies of target substance CAS NO 59-50-7 reviewed for genetic toxicity from reliable sources having Klimisch rating 2 and 4 considering weight of evidence approach.
The summary of the results are presented below
Sr. No |
End point |
Effect |
Species
|
Remarks |
1 |
Salmonella/mammalian microsome test |
negative with and without S9 metabolic activation
|
TA 1585, TA 100, TA 1537, and TA |
Data is from publication for Target chemical |
2 |
Bacterial reverse mutation assay (e.g. Ames test)
|
negative without metabolic activation |
TA 97, TA 100, TA 102, and TA 98
|
Data is from publication for Target chemical |
3 |
HGPRT assay |
negative with without metabolic activation |
Chinese hamster ovary
|
Data is from study report for Target chemical |
4 |
bacterial reverse mutation assay (e.g. Ames test) |
negative without metabolic activation |
TA 1535, TA 1537, TA 100 and TA 98
|
Data is from study report for Target chemical |
Based on the results summarized in above table for the target chemical CAS NO 59-50-7, it can be concluded that the substance chlorocresol is non-genotoxic.Thus chlorocresol is considered to be non-genetic toxic substance.
Justification for selection of genetic toxicity endpoint
4-chloro-3-methylphenol at doses 1.28, 6.4, 32, 160 and 800 ug per plate did not show mutagenic potential in Salmonella tester strain TA 1585, TA 100, TA 1537, and TA 98 with and without S9.
Justification for classification or non-classification
Chlorocresol can not be classified for genetic toxicity since, it failed to induce a mutagenic response.
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