(4-tert-butyl-2,6-dimethylphenyl)acetonitrile

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study performed according to a method similar to EU method C.2. Minor restrictions in design and reporting but outcome considered reliable and adequate for assessment.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Principles of method if other than guideline:

Based on EG Guideline 92/32/EEC.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

(4-tert-butyl-2,6-dimethylphenyl)acetonitrile

EC Number:

284-183-4

EC Name:

(4-tert-butyl-2,6-dimethylphenyl)acetonitrile

Cas Number:

84803-57-6

Molecular formula:

C14H19N

IUPAC Name:

2-(4-tert-butyl-2,6-dimethylphenyl)acetonitrile

Details on test material:

- Test substance: 2,6-Dimethyl-4-tert.-butylbenzylcyanid
- Aggregation state: white powder

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

The test substance is stirred in M4 medium for 20 hours and subsequently centrifuged for 20 minutes at 17700 G. A clear solution was the result.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna STRAUS, originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Nominal and measured concentrations:

- Nominal concentrations: 0 (control), 0.01, 0.1, 1, 10 and 100 mg/L.

Details on test conditions:

The test was carried out with an aqueous extract (leachate) of the test substance.
The effect is in the range of solubility.

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

15.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

Concentration-effect relationship after 48 hours:

Concentration (mg/L)	No of mobile daphnids (of 20)	Immobilisation (%)
0	19	5
0.01	19	5
0.1	19	5
1	17	15
10	11	45
100	5	75