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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study performed according to a method similar to EU method C.2. Minor restrictions in design and reporting but outcome considered reliable and adequate for assessment.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2002
Report date:
2002

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Principles of method if other than guideline:
Based on EG Guideline 92/32/EEC.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
(4-tert-butyl-2,6-dimethylphenyl)acetonitrile
EC Number:
284-183-4
EC Name:
(4-tert-butyl-2,6-dimethylphenyl)acetonitrile
Cas Number:
84803-57-6
Molecular formula:
C14H19N
IUPAC Name:
2-(4-tert-butyl-2,6-dimethylphenyl)acetonitrile
Details on test material:
- Test substance: 2,6-Dimethyl-4-tert.-butylbenzylcyanid
- Aggregation state: white powder

Sampling and analysis

Analytical monitoring:
no

Test solutions

Details on test solutions:
The test substance is stirred in M4 medium for 20 hours and subsequently centrifuged for 20 minutes at 17700 G. A clear solution was the result.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna STRAUS, originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Nominal and measured concentrations:
- Nominal concentrations: 0 (control), 0.01, 0.1, 1, 10 and 100 mg/L.
Details on test conditions:
The test was carried out with an aqueous extract (leachate) of the test substance.
The effect is in the range of solubility.

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
15.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

Concentration-effect relationship after 48 hours:

Concentration (mg/L)

No of mobile daphnids

(of 20)

Immobilisation (%)

0

19

5

0.01

19

5

0.1

19

5

1

17

15

10

11

45

100

5

75

Applicant's summary and conclusion