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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Description of key information

72h-EL50: 9.7 mg/L, 72h-EL10: 7.8 mg/l  (Loading rates, WAF study)

Key value for chemical safety assessment

EC50 for freshwater algae:
9.7 mg/L
EC10 or NOEC for freshwater algae:
7.8 mg/L

Additional information

The acute toxicity of Mandarin oil (Citrus Reticulata Blanco) ext. towards Pseudikirchneriella subcapitata was investigated according to OECD Guideline 201 under GLP. In view of the poor solubility in water, Water Accommodated Fractions were prepared. Algae were exposed to nominal loading rates (Water Accommodated Fractions (WAFs) of 1.0, 3.2, 10, 32 and 100 mg/l of the test substance and observed for 72 hours. Based on growth rate the 72h-ErL50 was 9.7 mg/l, the 72h-ErL10 was 7.8 mg/l, whereas the 72h-NOErL was 3.2 mg/l.


Remark: In this test of Harlan (2011) the concentration of limonene was measured. As the tested oil is a complex test substance that contains a range of constituents, the dose rates were prepared as Water Accommodated Fractions (WAF). The analytical measurements were used to establish the stability of the test solutions over time. They should not be used to express the toxicity of the Citrus Oils on the basis of a single substance or sum parameter.