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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1991-01-29 - 1991-08-01
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
GLP.
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
EEC, Part C: Method fot the determination of Ecotoxicity, C.1: "Acute Toxicity for Fish", No. L251/146, dated Sept. 19, 1984
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM
- Common name: Rainbow trout
- Source: Forellenhof Fredersloh, D-3413 Moringen 1
- Age at study initiation (mean and range, SD):
- Length at study initiation : first experiment: 6.30 +/- 0.84 cm (Mean +/- SD); second experiment: 6.08 +/- 0.82 cm (Mean +/- SD)
- Weight at study initiation : first experiment: 2.88 +/-1.19 g (Mean +/- SD); second experiment: 3.04 +/-1.53 g (Mean +/- SD)



ACCLIMATION
- Acclimation period: For at least 14 days in the laboratories of RCC and at least for the last 7 days to the test water and temperature.
- Acclimation conditions (same as test or not): yes
- Type of food: Commercial trout diet (Brutfutter FB50, Kronen-Fischkraftfutter, D-4320 Wesel)
- Feeding frequency: During acclimation until one day before the test



Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
2.5 mmol/l as CaCO3
Test temperature:
first experiment: 15.3°C - 17.1°C
second experiment: 15.8°C - 16.8°C
pH:
7.6 - 9.4
Dissolved oxygen:
8.3 mg/l or higher
Nominal and measured concentrations:
first experiment: 0, 5, 9, 15, 28, 50 mg/l (nominal)
second experiment: 0, 95, 171, 309, 555, 1000 mg/l (nominal)
Details on test conditions:

TEST SYSTEM
- Test vessel:
- Aeration: The test nedia were slightly areated during the test
- Type of flow-through (e.g. peristaltic or proportional diluter):
- No. of organisms per vessel: 10
- No. of vessels per control (replicates): 1

Test water without any additions.
CaCl2 x 2H2O : 294 mg/l
MgSO4 x 7H2O: 123 mg/l
NaHCO3: 65 mg/l
KCL: 5.8 mg/l
Water Hardness: 2.5 mmol/l as CaCO3
Alkalinity: 0.8 mmol/l
Ratio of Ca:Mg=4; Na:K=10:1



OTHER TEST CONDITIONS
- Adjustment of pH: Since in the test media of the concentrations 95-1000 mg/l, the pH values were in the range of pH 9.6-10.4, the pH values were adjusted with 1 mol HCL p.A. to pH 7.6-8.0
- Photoperiod: 16 hours per day
- Light intensity: abaout 50-300 Lux
During test duration the dissolved oxygen concentrations were in the test media always 8.3 mg/l or higher, the pH-values in the range of 7.6-9.4.

TEST CONCENTRATIONS

- Range finding study
- Test concentrations: 5, 9, 15, 28 and 50 mg/l

In the second experiment the test substance was weighed in concentrations of 95, 171, 309, 555 and 1000 mg/l and directly dissolved in each aquarium. Since the pH-values of these test media were in the range of pH 9.6 - 10.4, the pH values were adjusted with 1 mol HCl p.A. to 7.6 - 8.0 before introduction of the fish. Both experiments were performed including a control without any addition.
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
50 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
290 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 128-657 mg/l Confidence limit
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
555 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LOEC
Effect conc.:
95 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Mortality of control: no mortality wa observed during the test in the control

The lowest lethal concentration (LLC) was 309 mg/l.

In the control no mortality was observed during the test.

No remarkable observations were made at the behaviour of the test substance in the test media.

The test substance concentrations in the analysed media were in the range of 84.9 - 114.3 % of the nominal values during test duration. The test substance was sufficiently stable under test conditions. Therefore, all repoted results are related to the nominal concentrations of the test substance.

No mortalities occured in the first experiment in the nominal concentrations 5-50 mg/l.

Results of the second experiment:

Nominalconc.

(mg/l)

2 hours

24 hours

48 hours

72 hours

96 hours

Control 2

0 / 0

0 / 0

0 / 0

0 / 0

0 / 0

95

0 / 0

0 / 0

0 / 1

0 / 2

0 / 2

171

0 / 0

0 / 0

0 / 1

0 / 3

0 / 5

309

0 / 0

1 /9

1 / 5

2 / 10

7 / 10

555

0 / 0

9 / 10

10 / 10

- / -

- / - 

1000

0 / 0

10 / 10

- / -

- / -

- / -

LC50

--

414

368

340

290

95 % C.I.

-

349 - 491

302 -448

141 -822

128 -657

Validity criteria fulfilled:
yes
Remarks:
No mortalities occured in the control during the test. The dissolved oxygen concentrations in the test media were always 8.32 mg/l or higher. The test substance concentrations in the analysed test media were in the range of 84.9 - 114.3 % of the nominal
Conclusions:
In this study Dimethylaminopropyl methacrylamide was found to be toxic to rainbow trout after 96 h at concentrations of 95 mg/L (LOEC 96 h) and higher. The LC50 (96 h) was calculated to be 290 mg/L. The NOEC after 96 hours was 50 mg/L.
Executive summary:

In a 96-h acute toxicity study, rainbow trout (oncorhynchus mykiss) were exposed to Dimethylaminopropyl methacrylamide at nominal concentrations of 0 (control), 5, 9, 15, 28, 50, 95, 171, 309, 555, 1000 mg a.i./L under static/flow through conditions. The 96-h LC50 was 290 mg a.i./L. The NOEC value, based on mortality effects, was 50 mg a.i./L.

Based on the results of this study, Dimethylaminopropyl methacrylamide would be classified as not toxic to fish in accordance with GHS.

This toxicity study is classified as acceptable and satisfies the guideline requirements for acute toxicity to fish study. 

Results:

Test organism size: 6.08 +/- 0.82 cm

Type: Static 

LC50:  290 mg/L       95% C.I.:  128 - 657 mg /L

NOEC:  50 mg /L                            

Endpoint: mortality

Description of key information

The 96 h LC50 of in freshwater fish (Rainbow trout) was 290 mg/L (95% C.I.:  128 - 657 mg/L) mg/L according to OECD 203, static

conditions (RCC Umweltchemie GmbH & Co. KG, 1991) .

Key value for chemical safety assessment

LC50 for freshwater fish:
290 mg/L

Additional information

One acute freshwater study in fish is available for Dimethylaminopropyl methacrylamide which is consided reliable without restrictions (Klimisch score 1).

In a 96-h acute toxicity study, rainbow trout were exposed to Dimethylaminopropyl methacrylamide under static conditions according to OECD 203.

The 96-h LC50was 290 mg/L (95% CL (confidence limit): 128 - 657 mg/L, based on mortality). The 96 -h NOEC was 50 mg/L (based on mortality) (RCC Umweltchemie GmbH & Co. KG, 1991).