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EC number: 203-977-3 | CAS number: 112-49-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to reproduction: other studies
Administrative data
- Endpoint:
- toxicity to reproduction: other studies
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- Received: 26 August 1984
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Scientifically sound non-guideline study with restrictions.
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 985
- Report date:
- 1984
- Reference Type:
- publication
- Title:
- Unnamed
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Deviations:
- not applicable
- Principles of method if other than guideline:
- Triethylene glycol dimethyl ether was administered to femal mice for eight consecutive days and to pregnant mice from gestation days 7 to 14. MTD (maximum tolerated dose) was determined in non-pregnant mice and developmental toxicity was examined.
- GLP compliance:
- not specified
- Type of method:
- in vivo
Test material
- Reference substance name:
- 1,2-bis(2-methoxyethoxy)ethane
- EC Number:
- 203-977-3
- EC Name:
- 1,2-bis(2-methoxyethoxy)ethane
- Cas Number:
- 112-49-2
- Molecular formula:
- C8H18O4
- IUPAC Name:
- 2,5,8,11-tetraoxadodecane
- Reference substance name:
- Triethylene glycol dimethyl ether
- IUPAC Name:
- Triethylene glycol dimethyl ether
- Reference substance name:
- Triglyme
- IUPAC Name:
- Triglyme
- Details on test material:
- Test chemical was provided by NIOSH (National Institute for Occupational Safety and Health)
Constituent 1
Constituent 2
Constituent 3
Test animals
- Species:
- mouse
- Strain:
- CD-1
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Breeding Laboratories (Wilmington, Mass., USA)
- Age at study initiation:61-71 days
- Housing: 5 animals (MTD Study) or individually (Reproductive Study) per solid bottom cage with corn-cob bedding
- Diet (e.g. ad libitum): Wayne Lab Blox, ad libitum
- Water (e.g. ad libitum): tap water, ad libitum
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22-24°C
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on exposure:
- MTD Study:
5 Dose levels were tested with 10 mice in each dose group. Triethylene glycol dimethyl ether was administered by gavage at the same time each day for 8 consecutive days in 0.25 mL quantities. Treatment concentrations were based on the average animal weight on the day prior starting the dosing regime. Individual body weights were recorded on day 1 and day 8 of treatment, and on days 4 and 8 after the final dose (during recovery phase). Deaths were recorded cumulatively over the experimental period and necropsies were performed to determine if death was due to puncture of the esophagus during treatment.
Reproductive Study:
50 pregnant mice were randomly assigned to the treatment and control group. The mice were individually housed in separate cages. They were dosed for 8 consecutive days starting on day 7 of gestation. Treatment concentrations were based on the average day 6 dam weight. Maternal body weights were recorded on days 7 and 18 of gestation, and on day 3 postpartum. - Analytical verification of doses or concentrations:
- not specified
- Details on analytical verification of doses or concentrations:
- no data
- Duration of treatment / exposure:
- Day 7 to day 14 of gestation
- Frequency of treatment:
- daily
- Duration of test:
- Day 0 of gestation - day 3 postpartum
Doses / concentrations
- Remarks:
- Doses / Concentrations:
3500 mg/kg bw/d
Basis:
actual ingested
- No. of animals per sex per dose:
- MTD Study:
10 female mice
Dose levels: 445, 890, 1780, 3560 or 7120 mg/kg bw/d
Reproductive Study:
50 pregnant females
Dose level: 3500 mg/kg bw/d - Control animals:
- yes, concurrent vehicle
- Details on study design:
- Please refer to "Details on exposure"
- Statistics:
- The weights of the treated groups recorded in the MTD portion of the investigation were compared with the respective control weight using the general linear model with coded linear regressions. Toxicity values (LD50) and confidence intervals were determined using SAS probit analysis program. The reproductive index, maternal survival, and the total number of live pups per litter were analysed using chi-square analysis. Maternal weight gains over the gestational period were compared with the control using one-way analysis of variance.
Results and discussion
Effect levels
open allclose all
- Dose descriptor:
- other: LD50
- Effect level:
- 4 136.7 mg/kg bw/day (actual dose received)
- Sex:
- female
- Dose descriptor:
- other: MTD
- Effect level:
- 3 500 mg/kg bw/day (actual dose received)
- Sex:
- female
- Dose descriptor:
- NOAEL
- Effect level:
- < 3 500 mg/kg bw/day (actual dose received)
- Sex:
- female
- Basis for effect level:
- other: based on 97% survival of pregnant mice
- Dose descriptor:
- other: NOAEL (developmental)
- Effect level:
- < 3 500 mg/kg bw/day (actual dose received)
- Sex:
- female
- Basis for effect level:
- other: based on 100 % fetotoxicity
Observed effects
Any other information on results incl. tables
MTD Study
Diethylene glycol dimethal ether did not induce significant weight loss compared to the control body weight. Since the criterion of weight loss was not diagnostic for adult toxicity, MTD values were based on gross mortality. Using the cumulative mortality data, a dose was chosen for the MTD level that empirically exhibited a low level (LD10) of lethality. Moderate toxicity was noted for Ethylene glycol dimethyl ether. The MTD was derived at 2000 mg/kg bw/d.
Reproductive Study
Maternal lethality, as indicated by a reduction in percent maternal survival, was significant in the Diethylene glycol dimethyl ether group. The test item affected the reproductive index, a parameter reflecting severe prenatal lethality, significantly. In fact, none of the pregnant mice delivered any viable pups.
Table1: Effects of Treatment on Mortality and Weight in Non-Pregnant Mice
Compound |
Dose |
Cumulative |
Average weight |
|
|
|
|
Distilled Water |
- |
0/3 |
23.56±1.06 |
|
|
|
|
Ethylene glycol |
335 |
0/0 |
25.51±0.64 |
|
670 |
0/0 |
25.31±0.50 |
|
1340 |
0/0 |
25.68±0.67 |
|
2680 |
1/0 |
23.66±0.53 |
|
5360 |
10/0 |
- |
|
|
|
|
1 Represented as number dead due to treatment/number dead by esophageal puncture
2 Average weight of animals on day 8 of treatment (±SE)
Table2: Effect of Compound Exposure during Pregnancy on Maternal Survival and Weight Gain
Compound |
Dose |
Total |
%Maternal |
Weight gain |
|
|
|
|
|
Distilled |
- |
43 |
100 |
19.89±3.26 |
|
|
|
|
|
Ethylene glycol |
3000 |
45 |
603 |
- |
|
|
|
|
|
1 Comparison of the number of pregnant surivors with the total number pregnant (determined by treatment of uteri with 1% Sodium sulfide
2 Average increase in dams which produced litters (day 7 to day 18 ± SE)
3 Statistically significant at p<0.005
Applicant's summary and conclusion
- Conclusions:
- Based on the observed mortality and fetolethality the NOAEL (maternal, developmental) in mice is considered to be smaller than 3500 mg/kg bw/d. The MTD (8 daily applications) in female non-pregnant mice was set at 3500 mg/kg bw/d.
- Executive summary:
Triethylene glycol dimethyl ether was administered to female mice for eight consecutive days and to pregnant mice from gestation days 7 to 14. MTD (maximum tolerated dose) was determined in non-pregnant mice and developmental toxicity was examined.
MTD Study
Diethylene glycol dimethyl ether did not induce significant weight loss compared to the control body weight. Since the criterion of weight loss was not diagnostic for adult toxicity, MTD values were based on gross mortality. Using the cumulative mortality data, a dose was chosen for the MTD level that empirically exhibited a low level (LD10) of lethality. Moderate toxicity was noted for Triethylene glycol dimethyl ether. The MTD was derived at 3500 mg/kg bw/d.
Reproductive Study
Maternal lethality, as indicated by a reduction in percent maternal survival, was significant in the Triiethylene glycol dimethyl ether group (3500 mg/kg bw/d). The test item affected the reproductive index, a parameter reflecting severe prenatal lethality, significantly. In fact, none of the pregnant mice delivered any viable pups.
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