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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

dermal absorption in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
Received: 16 June 1998
Rationale for reliability incl. deficiencies:
other: Principle aspects are in line wtih the current OECD guideline but several deviations from guideline.

Data source

Reference Type:
Report date:

Materials and methods

Test guideline
equivalent or similar to guideline
OECD Guideline 428 (Skin Absorption: In Vitro Method)
one concentration, skin not rinsed at termination
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Bis(2-methoxyethyl) ether
EC Number:
EC Name:
Bis(2-methoxyethyl) ether
Cas Number:
Molecular formula:
Constituent 2
Reference substance name:
Constituent 3
Reference substance name:
Diethylene glycol dimethyl ether
Diethylene glycol dimethyl ether
Details on test material:
Supplier: Sigma Chemical Co., USA
Purity: 99%

Test animals

other: n.a.
Details on test animals or test system and environmental conditions:
The donors were men under 60 years of age.

Administration / exposure

Type of coverage:
other: in vitro study
unchanged (no vehicle)
Duration of exposure:
30, 60, 90, 120, 150, 180 and 240 min.
0.2 mL
No. of animals per group:
8 runs, one per determined time point
Control animals:
in vitro study
Details on study design:
Please refer to "Details on in vitro test system"
Details on in vitro test system (if applicable):
In vitro skin permeation using human skin was measured with the Franz method. A piece of full thickness human abdominal was excised at autopsy (within 24 hours post mortem), and immediately placed into palstic bags and stored in a freezer (-80°C), for a period of up to, but not exceeding, 4 months. This method of storage does not damage the barrier since no difference in permeability was observed between fresh and frozen segments of the same skin in a separate series of experiments. Before use, the skin was left to thaw gradually to room temperature, following which all subcutaneous fat was removed by scalpel. From specimen, 3x3 cm multiple pieces were cut and mounted individually on an eight-cell Franz diffusion assembly. The epidermal side if the skin was exposed to ambient conditions, while the dermal side was bathed in a physiological solution (15 mL volume). temperature was maintained at 32°C by circulating water through a jacket surrounding the chamber. After mounting, each piece of skin was allowed to stand for 2 hours before the experiments were begun. At time 0, a quantity of 0.2 mL Diehylene glycol dimethyl ether was placed on the epidermal surface. The area available for diffusion was 3.14 cm2. At selected intervals, 2 mL of the dermal bathing solution was removed and analysed. Each receptor sample was replaced with an equal volume of fresh physiological solution. The experiment was performed with eight cells at each of eight times (time 0, 30, 60, 90, 120, 150, 180 and 240 min), for a total of 64 samples.

Results and discussion

Signs and symptoms of toxicity:
not examined
in vitro study
Dermal irritation:
not examined
in vitro study
Absorption in different matrices:
Lag time: 36 +/- 3 min
Flux at steady state permeation: 0.952 +/- 0.340 mg/cm2/h

Permeation values of mixture (Diethylene glycol dimethyl ether 30% + acetone 70%)
Lag time: 49 +/- 28 min
Flux at steady state permeation: 0.674 +/- 0.305 mg/cm2/h
Total recovery:
not determined
Conversion factor human vs. animal skin:

Applicant's summary and conclusion

Results of the in vitro dermal absorption study performed with Diethylene glycol dimethyl ether:
The lag time was reported to be 36 +/- 3 min, the flux at steady state permeation was 0.952 +/- 0.340 mg/cm2/h.
Executive summary:

Due to the high structural similarity of Triethylene glycol dimethyl ether and Diethylene glycol dimethyl ether (difference: one ethyl group; but same functional groups) it is very likely that both compounds will show a similar skin penetration behaviour. Therefore, a read across from Triethylene glycol dimethyl ether to the data generated with Diethylene glycol dimethyl ether is justified to give a rough indication for the dimension of skin penetration properties. Furthermore, since the molecular weight of Triglyme (178.23 g/mol) is higher than that of Diglyme (134.18 g/mol), the substance is expected to be absorbed by the skin in a smaller amount than Diglyme.

An in vitro skin absorption study was performed applying Diethylene glycol dimethyl ether to dermatomed human skin. The lag time was reported to be 36 +/- 3 min, the flux at steady state permeation was 0.952 +/- 0.340 mg/cm2/h.