[29H,31H-phthalocyaninato(2-)-kappa4N29,N30,N31,N32]copper, sulfonated, condensed with 2,4- diaminobenzenesulfonic acid and ammonia, converted with sodium hydroxide 2,4,6-trichloro-1,3,5-triazine and ammonium chloride

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1987

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

Comparable to internationally accepted guidelines.

Deviations:

no

Principles of method if other than guideline:

Method A-11

GLP compliance:

no

Specific details on test material used for the study:

FAT 40045/C
Purity: 100 %

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

other: Zebrafish

Details on test organisms:

10 animals were used per concentration.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

23 °C

pH:

7.8 - 7.9

Dissolved oxygen:

8.6 - 8.7

Nominal and measured concentrations:

300 and 1000 mg/L

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

300 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

After 96 hours of exposure, 10/10 and 5/10 fish survived at 300 and 1000 mg/L, respectively.

Validity criteria fulfilled:

no

Conclusions:

The 96h LC50 of FAT 40045/C in this short-term fish toxicity test was determined to be 1000 mg/L.

Executive summary:

The short-term fish toxicity test was performed using a methodology equivalent or similar to OECD Guideline 203. Groups of fish (containing 10 each) were exposed to FAT 40045/C for 96 hours at following concentrations: 300 and 1000 mg/L After 96 hours of exposure, 10/10 and 5/10 fish survived at 300 and 1000 mg/L, respectively. Based on the findings of the study, the LC50 of FAT 40045/C in this short-term fish toxicity test was determined to be 1000 mg/L.

Description of key information

In the study designated as key, groups of zebrafish (containing 10 each) were exposed at test concentrations 300 or 1000 mg/L for a duration of 96 h. The number of surviving fish was recorded at 1, 4, 24, 48, 72 and 96 h. Mortality was observed only at the high concentration, with two fish dying within 48 h and 3 more within 96 h. Under the test conditions, the 96 and 48 h LC0 was 300 mg/L, whereas the 96 and 48 h LC50 and LC100 were both >1000 mg/L. The outcome of supporting studies available, also supports the conclusion that Reactive Blue 72 has low toxicity to fish when exposed for short term.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1 000 mg/L