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Short-term toxicity to aquatic invertebrates

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The short-term toxicity of alpha-Terpinyl Acetate to aquatic organisms was determined in a GLP-compliant OECD 202 guideline study. Daphnids (20/dose) were exposed for 48 hours to nominal concentrations of 1.2 to 18 mg/L under flow-through conditions with 0.1 mL/L dimethylformamide as solvent. The test concentrations were analytically verified and determined at 0.96 to 10 mg/L (51 to 80% of nominal concentrations). All daphnids in the control, solvent control and at the 0.96, 1.5 and 2.3 mg/L dose levels appeared normal throughout the test, with no immobility or overt signs of toxicity observed. Observations of lethargic daphnids were made for both the 5.2 and 10 mg/L treatment groups, with all surviving daphnids in the 10 mg/L treatment group noted as lethargic at test termination. Observations of daphnids trapped on the water surface were made at all test concentrations during the test. However, comparable observations of daphnids trapped on the surface were made in the control groups, and this observation was not considered to be a treatment-related effect. At the 10 mg/L dose level, 25% of the test organisms were immobile. Test conditions were maintained constant throughout the test, the dissolved oxygen concentration has been at least 80% of the air saturation value throughout the test. The 48-h EC50 value based on mean measured concentrations is >10 mg/L.

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