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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in mammalian cells
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: only one concentration was tested which also induced excessive cytotoxicity, this result is of little relevance for human health risk assessment purposes

Data source

Reference
Reference Type:
publication
Title:
Mutagenicity of metal cations in cultured cell from Chinese hamster
Author:
Miyaki, M. et al.
Year:
1979
Bibliographic source:
Mutat. Res. 68, 259-263

Materials and methods

Principles of method if other than guideline:
Induction of hprt mutations in V79 cells treated with cobalt chloride. Treatment was for 20 hr in the absence of metabolic activation. Only one concentration of cobalt chloride was tested.
GLP compliance:
no
Type of assay:
mammalian cell gene mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Cobalt dichloride
EC Number:
231-589-4
EC Name:
Cobalt dichloride
Cas Number:
7646-79-9
Molecular formula:
CoCl2
IUPAC Name:
cobalt(2+) dichloride
Details on test material:
Purity >99%no further details reported

Method

Target gene:
hprt
Species / strain
Species / strain / cell type:
Chinese hamster lung fibroblasts (V79)
Details on mammalian cell type (if applicable):
EMEM, 10% fetal calf serum 110µg/mL pyruvate
Additional strain / cell type characteristics:
not specified
Metabolic activation:
without
Test concentrations with justification for top dose:
0.2 mM
Vehicle / solvent:
water
Controls
Untreated negative controls:
yes
Negative solvent / vehicle controls:
no
True negative controls:
no
Positive controls:
yes
Positive control substance:
N-ethyl-N-nitro-N-nitrosoguanidine
Remarks:
10, 25 µM
Details on test system and experimental conditions:
Treatment was for 20 hr in the absence of metabolic activation. Only one concentration of cobalt chloride was tested (0.2 mM) and the nature of the salt was not given. This concentration induced a significant amount of toxicity (almost 90%) and induced 2.3-fold increase in hprt mutant frequency.
Evaluation criteria:
not stated
Statistics:
not stated

Results and discussion

Test results
Species / strain:
Chinese hamster lung fibroblasts (V79)
Metabolic activation:
without
Genotoxicity:
positive
Remarks:
2.3fold
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
88.7%
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):positiveReference is considered of limited relevance for the hazard assessment due to reporting and experimental deficiencies.