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EC number: 939-714-0 | CAS number: -
The substance is a moderate skin irritant but according to classification criteria as defined in GHS and CLP not subject to classification. DNNSA as a 50% formulation in light petroleum oil is a severe primary eye irritant in rabbits.
PRIMARY IRRITATION INDEX = ( sum of individual totals, each divided by 6 ) divided by 4 = 4.04
Scoring of Irritation
Evaluation of Skin Reaction
Erythema and eschar formation:
Very slight erythema (barely perceptible)
Moderate to severe erythema
Severe erythema (beet redness) to slight eschar formation (injuries in depth)
Very slight edema (barely perceptible)
Slight edema (edges of area well defined by definite raising)
Moderate edema (raised approximately 1 mm)
Severe edema (raised more than 1 mm) and extending beyond the area of exposure
A 50% DNNSA formulation in light paraffinic petroleum oil was tested on six healthy mature male albino rabbits (New Zealand variety) for evaluation of primary irritation. Prior to application of the sample, the backs were clipped free of hair. The skin was left intact at six test sites and was abraded on the other six. 0.5 ml of a liquid test material was applied to an area of skin approximately 1 inch square on each rabbit. Patches of surgical gauze measuring 1 inch square, 2 single layers thick, were applied over the samples and covered with a non-reactive tape. The rabbits were then wrapped with a rubber dam. After the 24-hour exposure, the dam and tape were removed and the skin reactions evaluated. An evaluation was also made at 72 hours after application. The reactions were scored according to the methods described in the Federal Hazardous Substances Act.
The primary irritation index was 4.04 indicating moderate irritation.
See detailed results and scoring procedure in attached document (next section).
Synex 1052, a 50% DNNSA formulation in light petroleum oil was tested for primary eye irritation in New Zealand White rabbits.
One tenth of a milliliter of test material was instilled in one eye of each rabbit. Six rabbits were treated. The other eye of each rabbit remained untreated and served as a control. The treated eyes were not washed. The rabbits were immobilized in head stocks for 24 hours and then returned to their cages. Ocular lesions were evaluated at 24, 48 and 72 hours and at 4, 7 and 14 days after treatment.
No clinical signs of adverse systemic effects were observed. The test material is a severe primary eye irritant.
Whereas DNNSA was shown to be a moderate skin irritant, not fulfilling classification criteria under GHS/CLP, it was considered causing serious damage to eyes and within 14 days hardly any reversibility was observed.
Justification for selection of skin irritation / corrosion endpoint: Scientifically sound study following acceptable testing procedure. Justification for selection of eye irritation endpoint: Scientifically sound study following acceptable testing procedure. Effects on eye irritation: highly irritating
According to criteria in CLP (regualtion EC No 1272/2008) DNNSA is considered not skin irritant but having irreversible efffects to eyes (Eye Damage Categroy 1).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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