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Toxicity to terrestrial plants

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Endpoint:
toxicity to terrestrial plants: long-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2011-08-17 to 2011-09-08
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
no
Vehicle:
no
Details on preparation and application of test substrate:
- Method of mixing into soil: The required amounts of test substance were weighed and mixed with 14.0 g quartz sand. These mixtures were added to containers filled with 1456 g soil. The soil and the quartz sand - test substance mixtures were thoroughly mixed with a mixer to ensure a homogenous distribution of the test substance in soil.
- Controls: Controls were prepared in the same manner as the vessels containing test substance.
Species:
Avena sativa
Plant group:
Monocotyledonae (monocots)
Species:
Brassica napus
Plant group:
Dicotyledonae (dicots)
Species:
Vicia sativa
Plant group:
Dicotyledonae (dicots)
Test type:
seedling emergence toxicity test
Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
no
Total exposure duration:
21 d
Test temperature:
19.6 – 20.7 °C
pH:
6.8 ± 0.2
Moisture:
By addition of demineralized water to the mixture the water content was adjusted to 40 % of the water holding capacity.
Details on test conditions:
TEST SYSTEM
- Testing facility: BASF SE
- Test container (type, material, size): PVC plant pots with an upper internal diameter of about 95 mm, covered by plastic dishes until the start of emergence, volume approx. 250 mL
- Amount of soil: 360 g
- Sowing depth: 0.5 cm for Brassica napus, 1.0 cm for Vicia sativa, 1.5 cm for Avena sativa
- Loading (plants/pot): 10 dry seeds, not pre-germinated
- No. of seeds per concentration: 40
- Number of replicates per concentration: 4
- Number of replicates for the control: 4
- Reduction of the seedlings to 5 plants: 22 Aug 2011

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: Germany/Rheinland-Pfalz/Offenbach, "Im Bildgarten", Nr. 508/2
- Pesticide use history at the collection site: The soil had not been treated with crop protection products for >4 years. No fertilizer had been applied during the last 5 years before sampling the soil.
- Collection conditions: Cloudy with an air temperature approx. 19°C
- Sampling depth (cm): Approx. 20 cm
- Sampling quantitiy: Approx. 200 kg
- Standard soil: Type 2.3
- Soil type: Silty sand (uS) according to German DIN ISO 11277; sandy loam according to USDA
- Organic carbon (%): 0.94 ± 0.10 %
- Maximum water holding capacity: 35.6 ± 3.0 g/100 g DM
- CEC: 10.7 ± 1.4 meq/100 g
- Pretreatment of soil: Drying for 4 days at room temperature, Pre-sieving with 10 mm screen, Last sieving 2 mm screen, Dry matter at packing time: 92.8%
- Storage (condition, duration): The soil was stored in a cold storage room in the building of the test facility in a closed plastic bag at a temperature of 5.9 – 7.0 ° C from 27 Jul 2011 up to 15 Aug 2011. From this date the soil was stored in the laboratory at room temperature.

GROWTH CONDITIONS
- Photoperiod: Day/night 16 hours light, 8 hours darkness
- Light intensity and quality: 7370 Lux ± 8 %
- Relative humidity (%):
- Watering regime and schedules: Daily with demineralized water

EFFECT PARAMETERS MEASURED: emergence and growth

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
Nominal and measured concentrations:
Nominal concentrations: 0, 15.7, 31.3, 62.5, 125 and 250 mg/kg dry weight
Reference substance (positive control):
no
Species:
Brassica napus
Duration:
22 d
Dose descriptor:
NOEC
Effect conc.:
15.7 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks on result:
other: Parameter: dry weight
Species:
Brassica napus
Duration:
22 d
Dose descriptor:
NOEC
Effect conc.:
125 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Species:
Brassica napus
Duration:
22 d
Dose descriptor:
EC50
Effect conc.:
146 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks on result:
other: Parameter: dry weight
Species:
Avena sativa
Duration:
22 d
Dose descriptor:
NOEC
Effect conc.:
125 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks on result:
other: Parameters: dry weight, shoot length
Species:
Avena sativa
Duration:
22 d
Dose descriptor:
NOEC
Effect conc.:
250 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Species:
Avena sativa
Duration:
22 d
Dose descriptor:
EC50
Effect conc.:
> 250 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks on result:
other: Parameters: dry weight, shoot length
Species:
Vicia sativa
Duration:
22 d
Dose descriptor:
NOEC
Effect conc.:
31.3 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks on result:
other: Parameters: dry weight, shoot length
Species:
Vicia sativa
Duration:
22 d
Dose descriptor:
NOEC
Effect conc.:
250 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Species:
Vicia sativa
Duration:
22 d
Dose descriptor:
EC50
Effect conc.:
206 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks on result:
other: Parameter: dry weight
Details on results:
SEEDLING EMERGENCE
The emergence rates of the seeds were determined in a preliminary test:
- Emergence rate of oats (Avena sativa) : 90 % after 7 days
- Emergence rate of oilseed rape (Brassica napus) : 80 % after 7 days
- Emergence rate of vetch (Vicia sativa) : 80 % after 7 days
Endpoint:
toxicity to terrestrial plants: long-term
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
See 'Attached justification'
Reason / purpose:
read-across source
Key result
Species:
Brassica napus
Duration:
22 d
Dose descriptor:
NOEC
Effect conc.:
15.7 mg/kg soil dw
Remarks on result:
other: s determined from read-across to C13 alcohol, branched

Description of key information

Toxic effects have been observed in studies with terrestric plants.

Key value for chemical safety assessment

Additional information

For Isotridecanol, one of the components of the C13 -15 -branched an linear alcohol, data is available from a study according to OECD 208 and in compliance with GLP criteria (BASF SE, 2011). In this long-term terrestrial plant study, 3 plant species (A. sativa, B. napus, V. sativa) were exposed to the test substance at nominal concentrations of 0 (control), 15.7, 31.3, 62.5, 125 and 250 mg/kg. The required amounts of test substance were weighed and mixed with an appropriate amounts of quartz sand and this mixture added to soil. Seedling emergence and growth were recorded throughout the test. From this study the lowest chronic effect value is a 22-d NOEC of 15.7 mg/Kg soil dw (Brassica napus).