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Toxicity to soil macroorganisms except arthropods

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toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2011-01-26 to 2011-06-16
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
according to
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
Details on preparation and application of test substrate:
- Controls: For preparation of each the test substance concentrations an amount of about 2550 g test substrate (equal to 2500 g dry weight of test substrate) was filled into a box and 825 mL of demineralized water was mixed with the soil. The four replicates per concentration were prepared separately by blending 755 g of moistened substrate with a mixture of quartz sand and test substance. The replicates for the control were prepared in the same manner, but without test substance. For each test replicate (four per test concentration) the calculated amount of test substance for about 565 g soil (555 g dry weight of soil and 10g quartz sand) was pulverized in a mortar and mixed well with 10 g quartz sand. The control replicates contained only test substrate and quartz sand.
Test organisms (species):
Eisenia fetida
Animal group:
Details on test organisms:
- Common name: Earthworm
- Source: Regenwurmfarm Tacke, Klosterdiek 61, 46325 Borken, Germany
- Age at test initiation: approx. 7 months (information from the supplier)
- Weight at test initiation: 352 mg – 433 mg (mean value per replicate/worm) and a clear visible clitellum were used for the test

- Acclimation period: Stored in test substrate at test conditions one day before start of exposure
Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
Total exposure duration:
14 d
Test temperature:
20.7 – 20.9 °C
pH value of the dry test substrate: 6.5 (mean value of 2 single values)
- Water content at test start: 35.2 % (mean of 2 single determinations: 35.3 % and 35.1 %))
- Water content of the test substrate at the end of the exposure period: 34.5 % of dry weight (single measurement)
Details on test conditions:
- Test container: Preserving jars, volume 1 L, covered with glass lids
- Amount of soil or substrate: 750 ± 1 g artificial soil (wet weight)
- No. of organisms per container: 10
- No. of replicates per treatment group: 4 test vessels per concentration
- No. of replicates per control: 4

- Composition (if artificial substrate): According to guideline
- Determination of water content of the dry test substrate: 2 % (mean of 2 single determinations: 2.1 % and 1.9 %)

- Photoperiod: Continuous illumination
- Light intensity: 556 Lux, (mean of 5 single measurements: 703, 504, 548, 509, 516 Lux)

EFFECT PARAMETERS MEASURED: Mortality, change in biomass

- Spacing factor for test concentrations: 2
Nominal and measured concentrations:
Nominal concentrations: 62.5, 125, 250, 500 and 1000 mg/kg dw
Reference substance (positive control):
14 d
Dose descriptor:
Effect conc.:
64.5 mg/kg soil dw
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
Remarks on result:
other: 95% CL: 48.7 - 77.0 mg/kg dw
Details on results:
- Total mass of adults at beginning of test: 384.3 to 422.4 mg (mean values)
- The mortality of the earthworm in the control group did not exceed 10 % at the end of exposure.
- The loss of biomass of the earthworms in the control group was 2.4 % at the end of exposure. In conclusion the test was considered to be valid.
Results with reference substance (positive control):
- The results with the reference substance were valid
- Relevant effect levels: LC50 = 22 mg/kg dw


14 -d LC0: 17.2 mg/kg soild dw

14 -d LC100: 242 mg/kg soil dw.

toxicity to soil macroorganisms except arthropods: short-term
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
See 'Attached justification'
Reason / purpose:
read-across source
Key result
14 d
Dose descriptor:
Effect conc.:
64.5 mg/kg soil dw
Remarks on result:
other: as determined from read-across to C13 alcohol, branched

Description of key information

Toxic effects have been observed in studies with soil living organisms.

Key value for chemical safety assessment

Additional information

For Isotridecanol, one of the components of the C13 -15 -branched an linear alcohol, a short-term earthworm toxicity test is available which was performed according to OECD TG 207 and in compliance with GLP criteria (BASF SE, Evonik, 2011). In this study earthworms (E. fetida) were exposed to Isotridecanol at nominal concentrations of 0 (control), 62.5, 125, 250, 500 and 1000 mg/kg dw in artificial soil for 14 days. The test substrates consisted of moistened substrate (ca. 35% water content throughout the test) to which a mixture of quartz sand and test substance was added. During the 14-day exposure period the loss of biomass was monitored. From this study a 14-d LC50 of 64.5 mg/kg soil dw was determined.