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Short-term toxicity to aquatic invertebrates

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Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
Samples from test concentrations 0 (control), 0.313, 1.25, 2.5 and 10 mg/L were taken at t = 0h (start) and t = 48h (end). Samples were taken from parallel vessels without daphnids.
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
The test was performed with an aqueous extract (eluate) of the test substance. The test substance was stirred in M4 medium for about 20 hours at 20 ± 2 °C. Undissolved test substance was removed by centrifugation (about 20 min at about 17700 G). The nominal concentration of the eluate was 10 mg/L. The test concentrations were prepared by diluting this eluate with M4 medium.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Source: Institut National de Recherche Chimique Appliquee, France), cultured under standard laboratory conditions
- Age at study initiation: 2 - 24 hours
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
2.2 - 3.2 mmol/L
Test temperature:
18 - 22 °C
pH:
8.0 - 8.3
Dissolved oxygen:
8.5 - 8.8 mg/L
Nominal and measured concentrations:
- Nominal test concentrations: control, 0.313; 0.625; 1.25; 2.5; 5.0 and 10.0 mg/L (based on range-finding test)
- Recovery: In the nominal test concentration range of 1.25 to 10 mg/L the average recovery was 23.3% (calculated as the average over all measurements (t = 0h and 48h). For the 0.313 mg/L nominal test concentration, the recovery was below the detection limit (LoD). This concentration was not considered for calculation of the average recovery because the higher nominal test concentration of 1.25 mg/L showed no effect.
- Measured concentrations (as calculated from average recovery): n.a., 0.073, 0.146, 0.291, 0.583, 1.165. and 2.330 mg/L.
Details on test conditions:
TEST SYSTEM
- Test vessel: 20-mL flat bottom glass tubes.
- Fill volume: 10 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 animals/mL

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium is aerated for about 24 hours until saturation with oxygen is reached
- Alkalinity: 0.8 - 1.0 mmol/L (up to pH 4.3)
- Ca/mg ratio: about 4:1
- Conductivity: 550 - 650 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: pH and oxygen contents after 0 h in an additional replicate without daphnids and 48 h in replicate 1 of each tested concentration

OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 hours day : night
- Light intensity: diffuse light (1 - 8 µE·m-2·s-1)

EFFECT PARAMETERS MEASURED:
swimming ability after 0, 24 and 48h
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.391 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Reported statistics and error estimates:
Statistical calculation of the EC50 using the probit analysis (Finney DJ, 1971)

ADDITIONAL REPORTED EFFECT VALUES BASED

48 -h EC0: 0.291 mg/L

48 -h EC100: 1.17 mg/L

RESULST BASED ON NOMINAL CONCENTRATIONS:
48 -h EC0: 1.25 mg/L
48 -h EC50: 1.68 mg/L
48 -h EC100: 5.0 mg/L

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
See 'Attached justification'
Reason / purpose:
read-across source
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
0.391 mg/L
Remarks on result:
other: as determined from read-across to C13 alcohol, branched

Description of key information

Acutely very toxic to aquatic invertebrates.

Key value for chemical safety assessment

Additional information

High quality data is available for C13 branched alcohol (CAS 27458 -92 -0) which is one of the components of alcohols C13 -15 -branched and linear. The toxicity of C13 branched was investigated in a study according to OECD 202 and in compliance with GLP criteria (BASF SE, 2003). In this study groups of 20 animals (D. magna) were exposed to the test substance for 48 hours under static conditions. A nominal test concentration of 10 mg/L was prepared by adding the substance to test medium followed by stirring (ca. 20 hours) and centrifugation to remove undissolved test substance. This water accomodated fraction was further diluted with test medium to obtain nominal test concentrations of 0 (control), 3.13, 6.25, 12.5, 25, 50 and 100% of saturation. Presence of test substance was analytically verified and the average recovery for all sampled test concentrations was 23.3%. Measured concentrations were 0.073, 0.146, 0.291, 0.583, 1.165. and 2.330 mg/L (back-calculated based on the 23.3% recovery). Daphnid mobility was determined as swimming ability after 0, 24 and 48 hours exposure. No effect on mobility was seen up to a test concentration of 0.291 mg/L. At higher test concentrations statistically significant immobilisation was observed. The 48 -h EC50 value was determined at 0.391 mg/L (based on measured concentration).