Registration Dossier
Registration Dossier
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EC number: 266-357-1 | CAS number: 66422-95-5
Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Description of key information
2,4-Diaminophenoxyethanol dihydrochloride was mutagenic in vitro. It induced gene mutations in Salmonella typhimurium strain TA98 in the presence of metabolic activation, and chromosome aberrations and micronuclei in cultured human lymphocytes with or without metabolic activation. In mouse lymphoma L5178Y cells in vitro, statistically significant increases in gene mutations (HPRT) were observed in both experiements conducted, but the increase was small and dose-response was not obvious.
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (positive)
Genetic toxicity in vivo
Description of key information
The test substance did not induce micronuclei in bone marrow erythrocytes or DNA damage (measured by unscheduled DNA synthesis) in rat liver cells in vivo, although both assays showed slight effects in individual animals. The urine of rats treated topically, orally or intraperitoneally with 2,4-diaminophenoxyethanol showed no mutagenic activity.
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Additional information
2,4-Diaminophenoxyethanol dihydrochloride was mutagenic in vitro. It
induced gene mutations in Salmonella typhimurium strain TA98 in the
presence of metabolic activation, and chromosome aberrations and
micronuclei in cultured human lymphocytes with or without metabolic
activation. In mouse lymphoma L5178Y cells in vitro, statistically
significant increases in gene mutations (HPRT) were observed in both
experiements conducted, but the increase was small and dose-response was
not obvious. The test substance did not induce micronuclei in bone
marrow erythrocytes or DNA damage (measured by unscheduled DNA
synthesis) in rat liver cells in vivo, although both assays showed
slight effects in individual animals. In published literature, negative
results have been reported for 2,4-diaminophenoxyethanol for the
induction of reversion mutations in Salmonella typhimurium strains
TA1535, TA100, TA1537, TA1538 and TA98 with and without Aroclor 1254
induced rat liver activation system and for the induction of reverse
mutations in strain XV185-14C and gene conversions in strain D4 of the
yeast Saccharomyces cerevisiae. The urine of rats treated topically,
orally or intraperitoneally with 2,4-diaminophenoxyethanol showed no
mutagenic activity.
Justification for classification or non-classification
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
