Registration Dossier
Registration Dossier
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EC number: 239-491-3 | CAS number: 15471-17-7
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
Trout (not further specified), acute toxicity test, OECD 203, LC50 (96h) > 1000 mg/L, NOEC (96h) > 1000 mg/L
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 1 000 mg/L
Additional information
The acute toxicity to fish of the test substance was tested according to OECD Guideline 203 in a flow-through freshwater test with Rainbow trouts (not further specified) as test organisms (Heisler, 1990). The study was conducted under certificated GLP compliance. The test duration was 96 hours with substance concentrations of 100 mg/L as well as 1000 mg/L. No reference substance was used since it is not essential according to the guideline description. A temperature of 16 °C was held constant, the pH value was 7.4 at the beginning of the experiment. A water hardness of 4.5 °d with a Magnesium content of 4.2 mg/L, Calcium content of 18.0 mg/L and Calcium carbonate of 88 mg/L was determined at the same time point. During the test the pH values ranged from 7.4 to 7.6 (substance concentration: 100 mg/L) and 7.6 - 7.8 (substance concentration: 1000 mg/L). The average oxygen content was measured with 8.0 - 8.2 mg/L (substance concentrations: 100 mg/L and 1000 mg/L). The animals were observed daily for several times and mortalities and abnormal behaviour were recorded. All animals were inconspicuous and no animal died. During macroscopic autopsy no experimental-specific findings were observed. According to these result the LC50 as well as the NOEC is determined to be > 1000 mg/L. As conclusion, the test substance needs not to be classified as toxic to fish.
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