Nonamethylenediamine

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2012-08-27 to 2012-10-03

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material:
PHYSICO-CHEMICAL PROPERTIES
- Water solubility: 80 g/100 g

Analytical monitoring:

not specified

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia
- Strain: magna
- Source: Carolina Biological Supply Company (Burlington, NC)
- Age at study initiation (mean and range, SD): less than 24 hours old
- Feeding during test: daphnids were not fed

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

20.1-20.9 ºC

pH:

pH was recorded at the beginning, 24 and 48 hours after treatment. pH 5.16 (positive control) - 9.72 (NMDA 180 mg/L)

Dissolved oxygen:

Dissolved oxygen was recorded at the beginning, 24 and 48 hours after treatment: 4.8 - 6.7

Salinity:

not reported

Details on test conditions:

TEST SYSTEM
- Test vessel: glass tube
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: natural spring water

OTHER TEST CONDITIONS
- Photoperiod: 12 hours light/12 hours dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Temperature, pH and dissolved oxygen

TEST CONCENTRATIONS
- Range finding study: The concentration of 100 mg/l of the test item in natural spring water was used for the limit test. The concentrations used for the full study were based on the range finding test results showing that the lowest concentration to cause 100 % immobilisation was 180 mg/l and the highest concentration to cause 0 % immobilisation was 10 mg/l.
- Test concentrations: 180 mg/l, 90 mg/l, 40 mg/l, 20 mg/l and 10 mg/l

Reference substance (positive control):

yes

Remarks:

K2Cr2O7 (4.8 mg/l)

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

46.31 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks:

(including immobility)

Details on results:

In the limit test, using 100 mg/l of the test substance nonamethylenediamine (NMDA), the percentage of immobilised dapnids is 80 % at the end of the test.
As the percentage of immobilization exceeded 10 % at 48 hours after exposure, a full study was conducted. In the full study, using 180 mg/l, 90 mg/l, 40 mg/l, 20 mg/l and 10 mg/l of the test substance nonamethylenediamine (NMDA), the percentage of immobilization was 100 %, 70 %, 45 %, 20 % and 0 % at the end of the test.
Based on the probit analysis of the data, the EC50 value (probit probability = 0.500) with 95 % percent confidence (p = 0.95) was 46.308 mg/ L.

Results with reference substance (positive control):

The positive reference substance K2Cr2O7 at 4.8 mg/l caused 100 % immobilisation of daphnids at 24 hours and 48 hours after treatment.
In the negative control groups, the percentage of immobilized daphnids is 0 at 24 hours and 48 hours after treatment.
The dissolved oxygen concentration at the end of the study is higher than 3 mg/l in all the test and control vessels, indicating that the results are valid.

Reported statistics and error estimates:

The probit analysis was conducted using IBM SPSS Statistics 19

Validity criteria fulfilled:

yes

Conclusions:

Based on the above results, in the acute toxicity study using daphnia as test system under static conditions, the EC50 of the test item nonamethylenediamine (NMDA) is 46.308 mg/ L .

Description of key information

Based on a reliable study according to OECD 202 using daphnia as test system under static conditions, the EC50 of the test item NMDA is 46.308 mg/ L .

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

46.31 mg/L