Sulfonium compounds, C11-14-alkylbis(hydroxyethyl), 2-hydroxyethyl sulfates (salts)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study according to German national standards.

Qualifier:

according to guideline

Guideline:

other: DIN 38412; part 15

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution of 1g/L was prepared in destilled water by adding the test material to the water. The test concentrations were achieved by dilution of the stock solution.
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): the test solution was clear up to the 4.64 mg/L treatment. Starting from the 10 mg/L treatment the test solution was turbid.

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Source: Fischzucht Eggers, Hohenwestedt, Germany
- Length at study initiation (length definition, mean, range and SD): 4-6 cm

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

20 °C

pH:

7.7 - 8.0

Dissolved oxygen:

7.3 - 8.6 mg/L

Nominal and measured concentrations:

Based on the results of a range finding study, the following concentrations (nominal) were tested: 0, 2.15, 4.64, 10.0, 21.5, and 46.4 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 10 L aquaria
- Aeration: weak
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
- Culture medium different from test medium: no
- Intervals of water quality measurement: pH O2 content and temperature were measured after 1,4,24,48,72 and 96h.

EFFECT PARAMETERS MEASURED: Mortality and annormal symptoms were recorded after 1,4,24,48,72 and 96h

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

14.66 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: geometric mean calculation (0% effect at 10 mg/L and 100% at 21.5 mg/L)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

21.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

From the test concentration 10 mg/l onwards, the test solutions were cloudy.

Sublethal observations / clinical signs:

Nominal Dead fish after
conc. 1h 24h 48h 72h 96h
[mg/l]
-----------------------------------------------------------
2.15 0/10 0/10 0/10 0/10 0/10
4.64 0/10 0/10 0/10 0/10 0/10
10.00 0/10 0/10 0/10 0/10 0/10
21.50 0/10 10/10 10/10 10/10 10/10
46.40 10/10 10/10 10/10 10/10 10/10

control 0/10 0/10 0/10 0/10 0/10
-----------------------------------------------------------

Intoxication symptoms:
----------------------
no abnormalities

Validity criteria fulfilled:

not specified

Conclusions:

The test substance showed to be harmful to freshwater fish.

Description of key information

LC50(96h) = 14.6 mg/L (nominal) for Leuciscus idus (DIN 38412)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

14.6 mg/L

Additional information

The acute aquatic toxicity of the Sulfonium compound mixture was investigated in a guideline study according to DIN 38412, with the golden orfe (Leuciscus idus) as test organism (BASF 1987). After 96 hours of exposure the LC50 was approximately 14.6 mg/L nominal test substance concentration.