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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2010-08-03 to 2010-10-01
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Brixham test substance number 09-0187, Lot Number: DR43250709
- Purity test date: 11 August 2009
- Analytical purity: 95.7 % primary amine % of total amines

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: in the dark, at ambient temperature

Analytical monitoring:
yes
Details on sampling:
- concentrations in test solutions were measured in triplicate samples per test concentration:
* at 0 hours, samples were taken from excess test solutions
* at 72 hours, samples were taken from a single test replicate solution of each exposure (containing alga)
Vehicle:
no
Details on test solutions:
- primary stock solution: 10 mg/L (nominal concentration):
* prepared by dissolving 0.02 g in 2000 mL of culture medium
* stirred for 10 minutes using magnetic follower
* solution observed to be clear and colourless
- test solutions prepared by addition of aliquots of the primary stock solution to culture medium to final volumes of 1000 mL
- 100 mL of test solution was dispensed to each test and blank vessel
Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
- common name: unicellular green alga
- strain: CCAP 278/4
- source: laboratory cultures maintained under axenic conditions
- inoculum for test: 4 days old culture of the alga in the exponential growth phase: 0.345 mL with nominal cell density of 0.5 x 10^4 cells/mL
- culture grown in medium, under test conditions
- culture medium for maintenance and test: AAP medium
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Post exposure observation period:
not applicable
Hardness:
no data
Test temperature:
22 ± 2 °C
pH:
- initial pH values were 7.5 ± 0.5, except for 3.2 mg/L (pH = 8.1), 5.6 mg/L (pH = 8.2) and 10 mg/L (pH = 8.7)
- pH values were adjusted with 0.1M HCl to final pH values of 7.7, 7.7 and 7.8 for 3.2, 5.6 and 10 mg/L, respectively
- at the end of the test pH values ranged from 7.3 to 7.8
Dissolved oxygen:
no data
Salinity:
not applicable
Nominal and measured concentrations:
nominal concentrations: 0.056, 0.10, 0.32, 0.56, 1.0, 3.2, 5.6 and 10 mg/L
measured concentrations: arithmetic mean: NM for 0.056 to 0.56 mg/L, 1.2, 3.3, 5.6, 9.9 mg/L
Details on test conditions:
- test vessel: glass conical flasks of 250 mL
- type: closed with foam bungs
- test solution: 100 mL
- illumination: cool-white, approximately 6000 lux
- culture condition: under nominal orbital shaking at 160 rpm
- replicates: 6 replicate cultures of controls with algae, 3 replicate cultures of each test concentration (with algae), one blank treatment without algae for each test concentration and control
Reference substance (positive control):
no
Duration:
72 h
Dose descriptor:
other: EyC50
Effect conc.:
3.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks on result:
other: 95 % CL: 2.1-3.8 mg/L
Duration:
72 h
Dose descriptor:
other: ErC50
Effect conc.:
4.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 95 % CL: 4.2-4.6 mg/L
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Details on results:
- at end of test microscopic observations were made on samples taken from a single replicate of the control and each test substance concentration
- observed cell appeared normal
Reported statistics and error estimates:
- statistical software package used: PCADA
- differences in cell counts between control and test conditions were found significant at p < 0.05
- Dunnett's procedure was used for one-way analysis of variance in cell particle densities (significance level p < 0.05)
-
Validity criteria fulfilled:
yes
Conclusions:
Reliable study (Klimisch 1) performed according to OECD guideline 201 and under GLP conditions reporting an ErC50 = 4.4 mg/L for the unicellular green alga Pseudokirchneriella subcapitata.

Description of key information

ErC50 (72h) = 4.4 mg/L for Pseudokirchneriella subcapitata (OECD Guideline 201).

Key value for chemical safety assessment

EC50 for freshwater algae:
4.4 mg/L
EC10 or NOEC for freshwater algae:
0.1 mg/L

Additional information

The Klimisch 1 study by Hutchinson and Hurd (2010) performed a test at concentrations of 0.056, 0.10, 0.32, 0.56, 1.0, 3.2, 5.6 and 10 mg/L. Algae of the strain Pseudokircheneriella subcapitata (three replicate flasks per concentration) and six replicate controls were tested. The test was considered valid.