Propylidynetrimethanol, propoxylated, reaction products with ammonia

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2010-08-03 to 2010-10-01

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Brixham test substance number 09-0187, Lot Number: DR43250709
- Purity test date: 11 August 2009
- Analytical purity: 95.7 % primary amine % of total amines

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: in the dark, at ambient temperature

Analytical monitoring:

yes

Details on sampling:

- concentrations in test solutions were measured in triplicate samples per test concentration:
* at 0 hours, samples were taken from excess test solutions
* at 72 hours, samples were taken from a single test replicate solution of each exposure (containing alga)

Vehicle:

no

Details on test solutions:

- primary stock solution: 10 mg/L (nominal concentration):
* prepared by dissolving 0.02 g in 2000 mL of culture medium
* stirred for 10 minutes using magnetic follower
* solution observed to be clear and colourless
- test solutions prepared by addition of aliquots of the primary stock solution to culture medium to final volumes of 1000 mL
- 100 mL of test solution was dispensed to each test and blank vessel

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

- common name: unicellular green alga
- strain: CCAP 278/4
- source: laboratory cultures maintained under axenic conditions
- inoculum for test: 4 days old culture of the alga in the exponential growth phase: 0.345 mL with nominal cell density of 0.5 x 10^4 cells/mL
- culture grown in medium, under test conditions
- culture medium for maintenance and test: AAP medium

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Post exposure observation period:

not applicable

Hardness:

no data

Test temperature:

22 ± 2 °C

pH:

- initial pH values were 7.5 ± 0.5, except for 3.2 mg/L (pH = 8.1), 5.6 mg/L (pH = 8.2) and 10 mg/L (pH = 8.7)
- pH values were adjusted with 0.1M HCl to final pH values of 7.7, 7.7 and 7.8 for 3.2, 5.6 and 10 mg/L, respectively
- at the end of the test pH values ranged from 7.3 to 7.8

Dissolved oxygen:

no data

Salinity:

not applicable

Nominal and measured concentrations:

nominal concentrations: 0.056, 0.10, 0.32, 0.56, 1.0, 3.2, 5.6 and 10 mg/L
measured concentrations: arithmetic mean: NM for 0.056 to 0.56 mg/L, 1.2, 3.3, 5.6, 9.9 mg/L

Details on test conditions:

- test vessel: glass conical flasks of 250 mL
- type: closed with foam bungs
- test solution: 100 mL
- illumination: cool-white, approximately 6000 lux
- culture condition: under nominal orbital shaking at 160 rpm
- replicates: 6 replicate cultures of controls with algae, 3 replicate cultures of each test concentration (with algae), one blank treatment without algae for each test concentration and control

Reference substance (positive control):

no

Duration:

72 h

Dose descriptor:

other: EyC50

Effect conc.:

3.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: 95 % CL: 2.1-3.8 mg/L

Duration:

72 h

Dose descriptor:

other: ErC50

Effect conc.:

4.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 95 % CL: 4.2-4.6 mg/L

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

0.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

- at end of test microscopic observations were made on samples taken from a single replicate of the control and each test substance concentration
- observed cell appeared normal

Reported statistics and error estimates:

- statistical software package used: PCADA
- differences in cell counts between control and test conditions were found significant at p < 0.05
- Dunnett's procedure was used for one-way analysis of variance in cell particle densities (significance level p < 0.05)
-

Validity criteria fulfilled:

yes

Conclusions:

Reliable study (Klimisch 1) performed according to OECD guideline 201 and under GLP conditions reporting an ErC50 = 4.4 mg/L for the unicellular green alga Pseudokirchneriella subcapitata.

Description of key information

ErC50 (72h) = 4.4 mg/L for Pseudokirchneriella subcapitata (OECD Guideline 201).

Key value for chemical safety assessment

EC50 for freshwater algae:

4.4 mg/L

EC10 or NOEC for freshwater algae:

0.1 mg/L

Additional information

The Klimisch 1 study by Hutchinson and Hurd (2010) performed a test at concentrations of 0.056, 0.10, 0.32, 0.56, 1.0, 3.2, 5.6 and 10 mg/L. Algae of the strain Pseudokircheneriella subcapitata (three replicate flasks per concentration) and six replicate controls were tested. The test was considered valid.