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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. certificate)
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material : AbI. Nr. 85-4113
- Analytical purity: 93.9 g/100 g; w(C6H1 1) = 25.4 g/100 g ; w(C3H60) = 68.5 g/100 g

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: at room temperature
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The reference substance was dissolved in demineralized water. Before adjusting the volume, a solution of internal standard (dissolved in demineralized water) was added. To an aliquot of the samples 0.5 ml of the internal standard were added.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
2.48 mM
Test temperature:
19.2 - 20.2 °C
pH:
7.9 - 9.3
Dissolved oxygen:
8.4 -8.9 mg/l
Nominal and measured concentrations:
Nominal test concentrations (mg/l): 3.13, 6.25, 12.5, 25, 50, 100, control
Analytical test concentrations (mg/l): 93 - 98 % recovery rate of the test substance in nominal concentrations of 100 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: flatt bottom glass tubes (20 ml)
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium according to ISO 10706
- Ca/mg ratio: 4:1
- Conductivity: 550 - 650 µS/cm


OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
- Light intensity: ca. 1 - 8 µE/(m²s) at a wave Iength of 400 - 750 nm


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 24 and 48 h
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
6.25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
13 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Cumulated immobility

Nominal conc. (mg/l)

cumulative immobility

24 h

48 h

0

0

0

3.13

0

0

6.25

0

2

12.5

1

6

25

18

20

50

19

20

100

19

20

Conclusions:
No immobilisation was observed during the 48-hour test period at 0 and 3.13 mg/L. The EC50 (48h) was determined to be 13 mg/L for Daphnia magna.

Description of key information

The test substance was demonstrated to have a EC50 (48h) of 13 mg/L for Daphnia magna. 

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
13 mg/L

Additional information

The Klimisch 1 study by BASF AG (2002) performed a short-term toxicity test at 3.13, 6.25, 12.5, 25, 50 and 100 mg/L. Twenty daphnids (4 replicates of 5 animals) were exposed to thes concentration and twenty other daphnids functioned as controls. No immobilisation was observed during the 48-hour test period at 0 and 3.13 mg/L. Immobilisation was observed at 6.25 mg/L (2/20 daphnids), 12.5 mg/L (6/20 daphnids), 25 mg/L (20/20 daphnids), 50 mg/L (20/20 daphnids) and 100 mg/L (20/20 daphnids).