Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2000-04-27 to 2000-06-01
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report Date:
2000

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid
Details on test material:
Name of test material: Trimethylolpropanepoly(oxypropylene)triamine
EC no.: 500-105-6
CAS no.: 39423-51-3
Physical state: clear colourless liquid
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 8T509 (Huntsman Polyurethanes, Belgium)
- Analytical purity: 90.1% primary amine, as a percentage of total amine

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: In the dark, at ambient room temperature

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge: Buckland Sewage Treatment Works, Newton Abbot, Devon, UK on 25 April 2000; predominantly domestic sewage treated at this facility
- Storage conditions: Kept aerated at room temperature and pH maintained at 7.0 +/- 1.0
- Storage length: 9 days
- Preparation of inoculum for exposure: Centrifuged, washed, and resuspended in test medium and solids concentration determined 7 days prior to test.
- Concentration of sludge: 30 mg/L
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Remarks:
BOD
Details on study design:
TEST CONDITIONS
- Composition of medium: Standard media
- Test temperature: 22 +/- 2 °C
- pH: 7.0 +/- 1.0
- pH adjusted: yes
- Continuous darkness: yes

TEST SYSTEM
- Number of culture flasks/concentration: 3
- Measuring equipment: Hach manometric biochemical oxygen demand apparatus- Dark 500 ml bottle on a magnetic stirrer connected to a closed end mercury manometer.
- Details of trap for CO2 and volatile organics if used: KOH in a seal cup in the neck of each bottle.

SAMPLING
- Sampling frequency: Oxygen uptake was recorded 5 times/week. Readings corrected for the blank and the volume of test solution.
Reference substance
Reference substance:
other: sodium acetate

Results and discussion

% Degradationopen allclose all
Parameter:
% degradation (O2 consumption)
Value:
< 5
Sampling time:
5 d
Parameter:
% degradation (O2 consumption)
Value:
< 5
Sampling time:
11 d
Parameter:
% degradation (O2 consumption)
Value:
< 5
Sampling time:
15 d
Parameter:
% degradation (O2 consumption)
Value:
< 5
Sampling time:
20 d
Parameter:
% degradation (O2 consumption)
Value:
< 5
Sampling time:
28 d

BOD5 / COD results

Results with reference substance:
The measured COD value of sodium acetate was 0.66 g O2 per g test substance. This is comparable to its expected value, and validates the technique. The maximum biodegradation level attained was 71%, 60% of which occurred within the 10 day-window as expected for a biodegradable substance, thus confirming that the activated sludge contained viable organisms.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
The test substance was not readily biodegradable under the conditions of the test.