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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1984-06-14 to 1984-07-11
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1984
Report date:
1984

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
EU Method B.1 (Acute Toxicity (Oral))
Deviations:
not applicable
Principles of method if other than guideline:
- Bliss, C.J., the statistics of bioassay academic press, New York, 1962
- Finney, D.J., Probit analysis, 2nd ed., Cambridge Univ. Press, Cambridge 1952
- Hunter, W.J.; Lingk, W.; Recht, P.: Intercomparison study on the determination of single administration toxicity in rats; Commission of the European communities, Heath and Safety Directorate, j. Assoc. Off. Anal. Chem. 62, 864 - 873, 1979
- Weber, E., Grundriß d. biol. Statistik, G. Fischer- Verlag, Stuttgart, 7. Auflage, 1972
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Potassium cyanate
EC Number:
209-676-3
EC Name:
Potassium cyanate
Cas Number:
590-28-3
Molecular formula:
KOCN
IUPAC Name:
potassium cyanate
Details on test material:
Potassium cyanate 98 = minimum 98 % Potassium cyanate; up to 2 % Potassium carbonate

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female
Details on test animals or test system and environmental conditions:
weight: male 158 -199 g; female 129 -177 g
age: male 55 - 62 days; female 63 - 73 days

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
water
Details on oral exposure:
MAXIMUM DOSE VOLUME APPLIED: 10 mL/kg

Doses:
A aqueous solution was prepared with a concentration of 100 mg/mL.
The volume of application was 4.64 - 10.0 mL/kg for male and for female resulting in 464 - 1000 mg/kg
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
Duration of observation period following administration: 14 days
- Frequency of observations: 30 min, 1, 2, 4, 8, 24 hours as well as on day 2 and thereafter daily
- Necropsy of survivors performed: yes

Results and discussion

Preliminary study:
NA
Effect levelsopen allclose all
Key result
Sex:
female
Dose descriptor:
LD50
Effect level:
567 mg/kg bw
Based on:
test mat.
95% CL:
>= 216 - <= 1 487
Key result
Sex:
male
Dose descriptor:
LD50
Effect level:
936 mg/kg bw
Based on:
test mat.
95% CL:
>= 278 - <= 3 151
Key result
Sex:
male/female
Dose descriptor:
LD0
Effect level:
464 mg/kg bw
Based on:
test mat.
Mortality:
The deaths occurred between 20 minutes and 4 hours after application.
Clinical signs:
The symptoms of poisoning were disorders of central nervous system, decrease of tonicity and loss of startle reflexes. Additional disorders of coordination of action, severe shiver and severe clonical convulsions was showed. Furthermore, tonic convulsions, arduous breathing and breathlessness were showed.
Body weight:
not indicated
Gross pathology:
not indicated
Other findings:
Macroscopic post mortem examination of the animals did not reveal any abnormalities.

Applicant's summary and conclusion

Interpretation of results:
Category 4 based on GHS criteria
Conclusions:
The acute oral LD50 value for potassium cyanate was determined to be 567 mg/kg bw in female rats and 936 mg/kg bw in male rats.
Executive summary:

In an acute oral toxicity study, groups of 55 to 73 days old Wistar rats (5/sex) were given a single oral dose of potassium cyanate (98 %.) in water at doses of 464 - 1000 mg/kg bw.

The acute oral LD50 value was determined to be 567 mg/kg bw in females and 936 mg/kg bw in males. The LD0 value for both sexes was 464 mg/kg bw.

The symptoms of poisoning were disorders of central nervous system, decrease of tonicity and loss of startle reflexes. Additional disorders of coordination of action, severe shiver and severe clonic convulsions was showed. Furthermore, tonic convulsions, arduous breathing and breathlessness were showed.

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