Potassium cyanate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1984-06-14 to 1984-07-11

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1984

Report date:

1984

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method B.1 (Acute Toxicity (Oral))

Deviations:

not applicable

Principles of method if other than guideline:

- Bliss, C.J., the statistics of bioassay academic press, New York, 1962
- Finney, D.J., Probit analysis, 2nd ed., Cambridge Univ. Press, Cambridge 1952
- Hunter, W.J.; Lingk, W.; Recht, P.: Intercomparison study on the determination of single administration toxicity in rats; Commission of the European communities, Heath and Safety Directorate, j. Assoc. Off. Anal. Chem. 62, 864 - 873, 1979
- Weber, E., Grundriß d. biol. Statistik, G. Fischer- Verlag, Stuttgart, 7. Auflage, 1972

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Potassium cyanate

EC Number:

209-676-3

EC Name:

Potassium cyanate

Cas Number:

590-28-3

Molecular formula:

KOCN

IUPAC Name:

potassium cyanate

Details on test material:

Potassium cyanate 98 = minimum 98 % Potassium cyanate; up to 2 % Potassium carbonate

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

weight: male 158 -199 g; female 129 -177 g
age: male 55 - 62 days; female 63 - 73 days

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Details on oral exposure:

MAXIMUM DOSE VOLUME APPLIED: 10 mL/kg

Doses:

A aqueous solution was prepared with a concentration of 100 mg/mL.
The volume of application was 4.64 - 10.0 mL/kg for male and for female resulting in 464 - 1000 mg/kg

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

Duration of observation period following administration: 14 days
- Frequency of observations: 30 min, 1, 2, 4, 8, 24 hours as well as on day 2 and thereafter daily
- Necropsy of survivors performed: yes

Results and discussion

Effect levels

Effect levels 1

Key result

Sex:

female

Dose descriptor:

LD50

Effect level:

567 mg/kg bw

Based on:

test mat.

95% CL:

>= 216 - <= 1 487

Effect levels 2

Key result

Sex:

male

Dose descriptor:

LD50

Effect level:

936 mg/kg bw

Based on:

test mat.

95% CL:

>= 278 - <= 3 151

Effect levels 3

Key result

Sex:

male/female

Dose descriptor:

LD0

Effect level:

464 mg/kg bw

Based on:

test mat.

Mortality:

The deaths occurred between 20 minutes and 4 hours after application.

Clinical signs:

other: The symptoms of poisoning were disorders of central nervous system, decrease of tonicity and loss of startle reflexes. Additional disorders of coordination of action, severe shiver and severe clonical convulsions was showed. Furthermore, tonic convulsions

Gross pathology:

not indicated

Other findings:

Macroscopic post mortem examination of the animals did not reveal any abnormalities.

Applicant's summary and conclusion

Interpretation of results:

Category 4 based on GHS criteria

Conclusions:

The acute oral LD50 value for potassium cyanate was determined to be 567 mg/kg bw in female rats and 936 mg/kg bw in male rats.

Executive summary:

In an acute oral toxicity study, groups of 55 to 73 days old Wistar rats (5/sex) were given a single oral dose of potassium cyanate (98 %.) in water at doses of 464 - 1000 mg/kg bw.

The acute oral LD50 value was determined to be 567 mg/kg bw in females and 936 mg/kg bw in males. The LD0 value for both sexes was 464 mg/kg bw.

The symptoms of poisoning were disorders of central nervous system, decrease of tonicity and loss of startle reflexes. Additional disorders of coordination of action, severe shiver and severe clonic convulsions was showed. Furthermore, tonic convulsions, arduous breathing and breathlessness were showed.