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Diss Factsheets

Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Meets basic scientific principles

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1963
Report date:
1963

Materials and methods

Principles of method if other than guideline:
5-10 mice/sex/dose were administered intraperitoneally different doses of the test substance and were observed for 14 days.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Suberonitrile
EC Number:
211-089-2
EC Name:
Suberonitrile
Cas Number:
629-40-3
Molecular formula:
C8H12N2
IUPAC Name:
octanedinitrile
Details on test material:
- Name of test substance (as cited in study report): Korksaeuredinitril
- Analytical purity: no data
- Substance No.: XIII/213
- Sample No.: C 520/326
- Physical state: liquid
- Density: 0.942 g/mL

Test animals

Species:
mouse
Strain:
other:
Sex:
male/female
Details on test animals or test system and environmental conditions:
No details reported.

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
other: Traganth
Doses:
1600, 200, 160 and 100 mL/kg bw (corresponding to approx. 1507, 188, 151 and 942 mg/kg bw; calculated assuming test substance density = 0.942 g/mL)
No. of animals per sex per dose:
5 - 10
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levelsopen allclose all
Sex:
male/female
Dose descriptor:
LD50
Effect level:
ca. 94 mg/kg bw
Based on:
test mat.
Remarks on result:
other: calculated assuming test substance density = 0.942 g/mL
Sex:
male/female
Dose descriptor:
LD50
Effect level:
100 mL/kg bw
Based on:
test mat.
Remarks on result:
other: original value
Mortality:
- 1600 mL/kg bw: 5/5 (within 1 hour)
- 200 mL/kg bw: 3/5 (within 1 day)
- 160 mL/kg bw: 9/10 (within 1 day)
- 100 mL/kg bw: 9/20 (4 within 1 hour, 5 within 1 day)
Clinical signs:
intermittent respiration, abdominal-lateral position, tremor, poor general condition
Body weight:
Mean weights at study initiation:
- Males: 27.8 g
- Females: 25.2 g
Gross pathology:
- Died animals: pale liver and kidney
- Sacrificed animals: no abnormalities

Applicant's summary and conclusion