Zeolite, cuboidal, crystalline, synthetic, non fibrous, thermally produced

Toxicity to terrestrial plants

Administrative data

Endpoint:

toxicity to terrestrial plants: long-term

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

2001-05-09 to 2001-11-08

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP guideline study, tested with the source substance CAS 1318-02-1. According to the ECHA Guidance Document "Practical guide 6: How to report read-across and categories (March 2010)", the reliability was changed from RL1 to RL2.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

Landesamt fpr Pflanzenbau und Pflanzenschutz, Rheinland-Pfalz, Germany

Test material

Test material information

Constituent 1

Reference substance name:

Automatically generated during migration to IUCLID 6, no data available

IUPAC Name:

Automatically generated during migration to IUCLID 6, no data available

Details on test material:

- Name of test material (as cited in study report): Doucil 4A (former Wessalith 4000)
- Related CAS number: 1318-02-1
- Framework: monoclinic
- Molecular formula: Na12[(AlO2)12(SiO2)12].27H2O
- Physical state: white powder
- Analytical purity: no data
- Lot/batch No.: 494
- Storage condition of test material: at room temperature, in closed package

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

no

Details on preparation and application of test substrate:

To avoid deviation effects due to resulting differences in organic matter of the soil, the test substance was mixed with the Standard Soil 3A instead of sand. Composition of the soil: 40% Standard Soil 3A (LUFA Speyer; organic carbon content 3.1%, 36.4% particles 0.02 mm, max. particle size 2 mm, density 1140 g/1000 mL, pH 7.1), 40% sand, 20% deionized water.

Test organisms

Species:

Raphanus sativus

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

purchased from a natural food store, belonging to the same size class

Study design

Test type:

other: seedling emergence and seedling growth test

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

no

Total exposure duration:

23 d

Test conditions

Test temperature:

20 +/- 2 °C

pH:

adjusted to 6.5 with HCl

Moisture:

60-75%

Nominal and measured concentrations:

Following concentrations were tested: 0, 0.5, 1.5, 5 and 15 g/kg.

Results and discussion

Effect concentrations

Effect concentrations 1

Species:

Raphanus sativus

Duration:

23 d

Dose descriptor:

EC50

Effect conc.:

4 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Effect concentrations 2

Species:

Raphanus sativus

Duration:

23 d

Dose descriptor:

LC50

Effect conc.:

5 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Effect concentrations 3

Species:

Raphanus sativus

Duration:

23 d

Dose descriptor:

NOEC

Effect conc.:

1 500 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

phytotoxicity

Any other information on results incl. tables

85% of the control seeds produced healthy seedlings exhibiting normal growth throughout the test.
EC50 for growth was determined as 4 g/kg, LC50 for emergence was 5 g/kg and NOEC growth was stated as 0.5 g/kg.

% weight per plant compared to control (mean 94.9 mg):
0.5       92% (mean 87.4 mg)
1.5       73% (mean 69.1 mg)
5         57% (mean 54.1 mg)
15         0% (mean 0)

% emergence compared to control (mean 85% emerged seeds):
0.5      100% (mean 85% emerged seeds)
1.5      100% (mean 85%)
5         65% (mean 55%)
15         0% (mean 0%)

At higher concentrations (15 g/kg), phytotoxic effects could be observed. Some of the seedlings showed a little emergence, but most of them could not unfurl even the cotyledon. If initial germination (until unfurling of the cotyledon) occurred completely, effect on growth seems to be a function of time only. Seeds in the control emerged significantly earlier than in treatments.
However, the effects seem to be due to the water availability within the soil. The soil with the test substance incorporated was very dense, i.e. the resulting mixture hardens strongly so that the seeds are not able to penetrate the soil. So the phyotoxic effects seem to depend on the physical properties of the test substance. At concentrations of 1.5 g/kg and below, no phytotoxic effects could be determined.