Sulisobenzone

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from peer reviewed journal

Justification for type of information:

Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

Inhibition concentration of test material on Vibrio fischeri

GLP compliance:

not specified

Analytical monitoring:

not specified

Details on sampling:

A stock solution of test material was prepared in water at a concentration of 1g/l.

Test organisms (species):

Vibrio fisheri

Details on inoculum:

Strain: NRRL-B-11177
Source: Dr. Lange GmbH, Düsseldorf, Germany

Test type:

static

Water media type:

freshwater

Total exposure duration:

30 min

Test temperature:

15 ± 0.2 °C

Details on test conditions:

The luminescence of bacteria was measured on a LUMIStox 300 luminometer

Duration:

30 min

Dose descriptor:

other: IC20

Effect conc.:

67.3 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

other: luminescence inhibition

Duration:

30 min

Dose descriptor:

IC50

Effect conc.:

301 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

other: luminescence inhibition

Remarks on result:

other: (95% confidence interval) 300–302 mg/l

Duration:

30 min

Dose descriptor:

other: IC80

Effect conc.:

1 350 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

other: luminescence inhibition

Details on results:

IC20, IC50 and IC80 values were calculated using a log-linear regression analysis and represents the concentration of a tested compound that is required for 20, 50 and 80% luminescence inhibition after 30 minutes of exposure.

Conclusions:

The 30 min inhibitory concentrations, IC20, IC50 and IC80 was observed to be 67.3 , 301 , 1350 mg/l respectively.

Executive summary:

20 , 50 and 80% luminescence inhibition of test organism Vibrio fischeri NRRL-B-11177 was evaluted after 30 minutes of exposure of test material. The luminescence of bacteria was measured on a LUMIStox 300 luminometer . The 30 min inhibitory concentrations, IC20, IC50 and IC80 values were calculated using a log-linear regression analysis. The 30 min inhibitory concentrations, IC20, IC50 and IC80 was observed to be 67.3 , 301 , 1350 mg/l respectively.

Description of key information

Toxicity to microorganisms:

20 , 50 and 80% luminescence inhibition of test organism Vibrio fischeri NRRL-B-11177 was evaluted after 30 minutes of exposure of test material. The luminescence of bacteria was measured on a LUMIStox 300 luminometer . The 30 min inhibitory concentrations, IC20, IC50 and IC80 values were calculated using a log-linear regression analysis. The 30 min inhibitory concentrations, IC20, IC50 and IC80 was observed to be 67.3 , 301 , 1350 mg/l respectively.

Key value for chemical safety assessment

EC50 for microorganisms:

301 mg/L

Additional information

Toxicity to microorganisms:

20 , 50 and 80% luminescence inhibition of test organism Vibrio fischeri NRRL-B-11177 was evaluted after 30 minutes of exposure of test material. The luminescence of bacteria was measured on a LUMIStox 300 luminometer . The 30 min inhibitory concentrations, IC20, IC50 and IC80 values were calculated using a log-linear regression analysis. The 30 min inhibitory concentrations, IC20, IC50 and IC80 was observed to be 67.3 , 301 , 1350 mg/l respectively.