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Ecotoxicological information

Toxicity to microorganisms

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Reference
Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from peer reviewed journal
Justification for type of information:
Data is from peer reviewed journal
Qualifier:
according to guideline
Guideline:
other: as mentioned below
Principles of method if other than guideline:
Inhibition concentration of test material on Vibrio fischeri
GLP compliance:
not specified
Analytical monitoring:
not specified
Details on sampling:
A stock solution of test material was prepared in water at a concentration of 1g/l.
Test organisms (species):
Vibrio fisheri
Details on inoculum:
Strain: NRRL-B-11177
Source: Dr. Lange GmbH, Düsseldorf, Germany
Test type:
static
Water media type:
freshwater
Total exposure duration:
30 min
Test temperature:
15 ± 0.2 °C
Details on test conditions:
The luminescence of bacteria was measured on a LUMIStox 300 luminometer
Duration:
30 min
Dose descriptor:
other: IC20
Effect conc.:
67.3 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
other: luminescence inhibition
Duration:
30 min
Dose descriptor:
IC50
Effect conc.:
301 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
other: luminescence inhibition
Remarks on result:
other: (95% confidence interval) 300–302 mg/l
Duration:
30 min
Dose descriptor:
other: IC80
Effect conc.:
1 350 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
other: luminescence inhibition
Details on results:
IC20, IC50 and IC80 values were calculated using a log-linear regression analysis and represents the concentration of a tested compound that is required for 20, 50 and 80% luminescence inhibition after 30 minutes of exposure.
Conclusions:
The 30 min inhibitory concentrations, IC20, IC50 and IC80 was observed to be 67.3 , 301 , 1350 mg/l respectively.
Executive summary:

20 , 50 and 80% luminescence inhibition of test organism Vibrio fischeri NRRL-B-11177 was evaluted after 30 minutes of exposure of test material. The luminescence of bacteria was measured on a LUMIStox 300 luminometer . The 30 min inhibitory concentrations, IC20, IC50 and IC80 values were calculated using a log-linear regression analysis. The 30 min inhibitory concentrations, IC20, IC50 and IC80 was observed to be 67.3 , 301 , 1350 mg/l respectively.

Description of key information

Toxicity to microorganisms:

20 , 50 and 80% luminescence inhibition of test organism Vibrio fischeri NRRL-B-11177 was evaluted after 30 minutes of exposure of test material. The luminescence of bacteria was measured on a LUMIStox 300 luminometer . The 30 min inhibitory concentrations, IC20, IC50 and IC80 values were calculated using a log-linear regression analysis. The 30 min inhibitory concentrations, IC20, IC50 and IC80 was observed to be 67.3 , 301 , 1350 mg/l respectively.

Key value for chemical safety assessment

EC50 for microorganisms:
301 mg/L

Additional information

Toxicity to microorganisms:

20 , 50 and 80% luminescence inhibition of test organism Vibrio fischeri NRRL-B-11177 was evaluted after 30 minutes of exposure of test material. The luminescence of bacteria was measured on a LUMIStox 300 luminometer . The 30 min inhibitory concentrations, IC20, IC50 and IC80 values were calculated using a log-linear regression analysis. The 30 min inhibitory concentrations, IC20, IC50 and IC80 was observed to be 67.3 , 301 , 1350 mg/l respectively.