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Ecotoxicological information

Long-term toxicity to fish

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Reference
Endpoint:
fish, juvenile growth test
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: from peer reviewed journal
Justification for type of information:
Data is from peer reviewed journal
Qualifier:
according to
Guideline:
other: Fish in vivo screening assay (OECD, 2004).
Principles of method if other than guideline:
Fish, juvenile growth test in vivo screening assay (OECD, 2004) was performed.
GLP compliance:
not specified
Analytical monitoring:
not specified
Vehicle:
yes
Details on test solutions:
Stock solutions were made in ethanol and stored in the dark at 4C.
Test organisms (species):
Pimephales promelas
Details on test organisms:
Juvenile, sexually undifferentiated fathead minnows (Pimephales promelas), between 2 and 3 months of age and with a total body length between 19 and 27 mm
Test type:
semi-static
Water media type:
freshwater
Total exposure duration:
14 d
Hardness:
total hardness 160 mg/l as CaCO3
pH:
7.2 - 7.9
Dissolved oxygen:
6.5–8.3 mg/l
Nominal and measured concentrations:
Nominal concentrations-10, 1000, 5000 ug/l.
Details on test conditions:
total alkalinity 30 mg/l as CaCO3; solvent control (SC, 1 ml ethanol in 10 liters of water) and positive control for estrogenic activity (100 ng/l E2)
Reference substance (positive control):
yes
Remarks:
100 ng/l E2 (17β-Estradiol)
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
1.048 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
other: body size
Remarks on result:
other: Actual value- 1048 µg/L
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
> 4.897 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
dissolved
Basis for effect:
mortality
Remarks on result:
other: Actual value- 4897 µg/L
Results with reference substance (positive control):
No mortality was observed in positive control
Conclusions:
Long term toxicity (14 d) value of test material to Pimephales promelas examined of NOEC:1.048 mg/l (on the basis of body size) and NOEC: >4.897 mg/l (on the basis of mortality).
Executive summary:

The 14-days fish experiments were conducted using juvenile, sexually undifferentiated fathead minnows (Pimephales promelas), between 2

and 3 months of age and with a total body length between 19 and 27 mm . Fish were held in well-aerated reconstituted tap water medium.

The studies were conducted using a 24-h static-renewal procedure with daily renewal of total aquaria water. For exposure, 10 randomly selected fish were each placed in stainless-steel tanks (10 liter) and exposed to individual UV filters for 14 days. Long term toxicity (14 d) value of test material to Pimephales promelas examined of NOEC:1.048 mg/l (on the basis of body size) and NOEC: >4.897 mg/l (on the basis of mortality). Based on the above effect concentration it is considered to be non - hazardous to aquatic environment and can not be classified as per CLP criteria .

Description of key information

Long term toxicity to fish:

The 14-days fish experiments were conducted using juvenile, sexually undifferentiated fathead minnows (Pimephales promelas), between 2

and 3 months of age and with a total body length between 19 and 27 mm . Fish were held in well-aerated reconstituted tap water medium.

The studies were conducted using a 24-h static-renewal procedure with daily renewal of total aquaria water. For exposure, 10 randomly selected fish were each placed in stainless-steel tanks (10 liter) and exposed to individual UV filters for 14 days. Long term toxicity (14 d) value of test material to Pimephales promelas examined of NOEC:1.048 mg/l (on the basis of body size) and NOEC: >4.897 mg/l (on the basis of mortality). Based on the above effect concentration it is considered to be non - hazardous to aquatic environment and can not be classified as per CLP criteria .

Key value for chemical safety assessment

EC10, LC10 or NOEC for freshwater fish:
4.897 mg/L

Additional information

Long term toxicity to fish:

The 14-days fish experiments were conducted using juvenile, sexually undifferentiated fathead minnows (Pimephales promelas), between 2

and 3 months of age and with a total body length between 19 and 27 mm . Fish were held in well-aerated reconstituted tap water medium.

The studies were conducted using a 24-h static-renewal procedure with daily renewal of total aquaria water. For exposure, 10 randomly selected fish were each placed in stainless-steel tanks (10 liter) and exposed to individual UV filters for 14 days. Long term toxicity (14 d) value of test material to Pimephales promelas examined of NOEC:1.048 mg/l (on the basis of body size) and NOEC: >4.897 mg/l (on the basis of mortality). Based on the above effect concentration it is considered to be non - hazardous to aquatic environment and can not be classified as per CLP criteria .