Sulisobenzone

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Justification for type of information:

Data is from from experimental study report

Qualifier:

according to guideline

Guideline:

other: DIN 38 412 "TEST PROCEDURE WITH WATER ORGANISMS (GROUP L) GENERAL INFORMATION FOR THE PLANNING, IMPLEMENTATION AND EVALUATION OF BIOLOGICAL TEST PROCEDURES (L1) "AND" DETERMINATION OF THE EFFECT OF WATER INGREDIENTS

Principles of method if other than guideline:

The method used closely followed the guideline of DIN 38 412 "Test procedure with water organisms (group l). General information The planning, implementation and evaluation of biological test procedures (l1) "and" determination of the effect of water on ingredients In fish - fish test (l15) ", june 1982 using a static procedure.

GLP compliance:

no

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Leuciscus idus

Details on test organisms:

Animal species : golden orfe (leuciscus idus l ., golden variety )
Body length : 9 .8 cm (range : 8 .5 - 11 .4)
Body weight : 9 .1 g (range : 5 .8 - 17 .0 )

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

Total hardness : about 2 .5 mmol/l

Test temperature:

Test temperature : 21 - 24 deg. Cwater temp. - 19 - 20 deg. C

pH:

pH : about 8 . 0

Dissolved oxygen:

Oxygen content : > 60% of maximum saturation

Nominal and measured concentrations:

Nominal concentrations- 46.4, 100, 215, 464 mg/L.

Details on test conditions:

No. Of animals per test concentration: 10The fish were kept in a flow-through tank in tap water cleaned by active carbon and aerated with oil-free air .

Reference substance (positive control):

yes

Remarks:

chloroacetamide

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

215 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 215 - < 464 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

416 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Results with reference substance (positive control):

LC50 after 48 h: about 31 mg/l

Conclusions:

Short term toxicity to fish Leuciscus idus for 96 hrs fo test chemical examined at effect level as NOEC: 215 mg/L and LC50: > 215 - <464 mg/L and LC100: 416 mg/L this all values indicate that the chemical dose not exhibit short term toxicity to fish.

Executive summary:

Short term toxicity to fish Leuciscus idus for 96 hrs for the test chemical was examined in accordance with method closely followed the guideline of DIN 38 412 "Test procedure with water organisms (group l). General information The planning, implementation and evaluation of biological test procedures (l1) "and" determination of the effect of water on ingredients In fish - fish test (l15) ", june 1982 using a static procedure.The fish were kept in a flow-through tank in tap water cleaned by active carbon and aerated with oil-free air . Nominal concentrations used were 46.4, 100, 215, 464 mg/L. Short term toxicity to fish Leuciscus idus for 96 hrs fo test chemical examined at effect level as  NOEC: 215 mg/L and LC50: > 215 - <464 mg/L and LC100: 416 mg/L this all values indicate that the chemical dose not exhibit short term toxicity to fish.

Description of key information

Short term toxicity to fish:

Short term toxicity to fish Leuciscus idus for 96 hrs for the test chemical was examined in accordance with method closely followed the guideline of DIN 38 412 "Test procedure with water organisms (group l). General information The planning, implementation and evaluation of biological test procedures (l1) "and" determination of the effect of water on ingredients In fish - fish test (l15) ", june 1982 using a static procedure.The fish were kept in a flow-through tank in tap water cleaned by active carbon and aerated with oil-free air .Nominal concentrations used were 46.4, 100, 215, 464 mg/L. Short term toxicity to fish Leuciscus idus for 96 hrs fo test chemical examined at effect level as  NOEC: 215 mg/L and LC50: > 215 - <464 mg/L and LC100: 416 mg/L this all values indicate that the chemical dose not exhibit short term toxicity to fish and can be considered to be not classiffied as per CLP criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

215 mg/L

Additional information

Short term toxicity to fish:

The effect of test material was observed on fish considering two experimental reports as mentioned below:

Short term toxicity to fish Leuciscus idus for 96 hrs for the test chemical was examined in accordance with method closely followed the guideline of DIN 38 412 "Test procedure with water organisms (group l). General information The planning, implementation and evaluation of biological test procedures (l1) "and" determination of the effect of water on ingredients In fish - fish test (l15) ", june 1982 using a static procedure.The fish were kept in a flow-through tank in tap water cleaned by active carbon and aerated with oil-free air .Nominal concentrations used were 46.4, 100, 215, 464 mg/L. Short term toxicity to fish Leuciscus idus for 96 hrs fo test chemical examined at effect level as  NOEC: 215 mg/L and LC50: > 215 - <464 mg/L and LC100: 416 mg/L this all values indicate that the chemical dose not exhibit short term toxicity to fish.

In another report , study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 12.5 mg , 25 mg/L, 50 mg/L, 100 mg/L, 200 mg/L & 400 mg/L of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 3.125 mg/L, 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L, respectively. 

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be 25 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria. The test substance is redialy degradable in aquatic environment and has low chances to be hazardous to aquatic environment . Hence , it can be considered to be not classified as per CLP criteria.