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Diss Factsheets
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EC number: 229-314-8 | CAS number: 6471-50-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Adsorption / desorption
Administrative data
- Endpoint:
- adsorption / desorption: screening
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- Report date: 2012-09-06
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Well documented study performed according to relevant guidelines. However not performed under GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
- Deviations:
- no
- GLP compliance:
- no
- Type of method:
- HPLC estimation method
- Media:
- soil/sewage sludge
Test material
- Reference substance name:
- 4-[(2,5-dichlorophenyl)azo]-3-hydroxy-N-phenylnaphthalene-2-carboxamide
- EC Number:
- 227-930-1
- EC Name:
- 4-[(2,5-dichlorophenyl)azo]-3-hydroxy-N-phenylnaphthalene-2-carboxamide
- Cas Number:
- 6041-94-7
- IUPAC Name:
- 4-[(2,5-dichlorophenyl)diazenyl]-3-hydroxy-N-phenyl-2-naphthamide
Constituent 1
- Radiolabelling:
- no
Study design
- Test temperature:
- Column temperature: 40 deg C
HPLC method
- Details on study design: HPLC method:
- Principle of the method:
HPLC is performed on analytical columns packed with commercially available cyanopropyl solid phase containing lipophilic and polar moieties. A moderately polar stationary phase based on silica matrix is used. While passing through the HPLC column along with the mobile phase the test substance interacts with the stationary phase. As a result of partitioning between mobile and stationary phases the test substance is retarded.
Procedure
The distribution coefficient was estimated by the HPLC method using isocratic elution. The HPLC system was calibrated for log k' versus log Koc using reference substances of known log Koc. Test substance was dissolved in tetrahydrofurane. Calibration solutions were prepared in acetonitrile.
Test substance solution: 12.1 mg in 50 ml tetrahydrofurane
Calibration solutions: approx. 5-10 µg/ml in acetonitrile
Dead time marker: sodium nitrate
Instrumental conditions
HPLC instrument: Agilent 1100
Injection volume: 5 μl
Reference substances see table under "any other informations on materials and methods inc. tables"
Detector: UV (DAD), 254 nm (test substance) and 220 nm (calibration)
Column: μBondapak CN, 150 mm x 3.1 mm, 10 μm
Mobile phase: water/acetonitril = 55/45 (v/v)
Flow rate: 1 ml/min
Column temperature: 40 deg C
Evaluation of results performed according to the guideline.
Results and discussion
Adsorption coefficient
- Type:
- log Koc
- Value:
- > 5
- Temp.:
- 40 °C
- Remarks on result:
- other: HPLC method: Reference of highest log Koc used: DDT (log Koc 5.63) - by linear extrapolation (R^2= 0.9869) a log Koc of 6.2 was derived for the test item. Column temperture 40 deg C
Results: HPLC method
- Details on results (HPLC method):
- Calibration line and evaluation:
From the calibration line log K' versus log Koc the following equation was calculated:
Y = 0.1522x-0.0768, with R^2 = 0.9869
For the test substance, k' was determined as 7.25, log k' = 0.86
From this, the log Koc of the test substance was estimated as follows:
log Koc = (log(k') - b)/a = [0.86 - (-0.0768)]/0.1522 = 6.2
The calculated log Koc value of the test substance is outside the calibrated range. 4,4'-DDT is the reference substance with highest log Koc value available. Therefore only an extrapolated value for the test substance can be obtained.
Any other information on results incl. tables
Analytical data:
Substance |
log Koc |
Inj. 1 |
Inj. 2 |
k' |
log k' |
mass on |
Sodium nitrate |
-- |
0.839 |
0.841 |
--- |
--- |
50 |
Acetanilide |
1.25 |
1.882 |
1.883 |
1.24 |
0.09 |
55 |
Phenol |
1.32 |
1.944 |
1.943 |
1.31 |
0.12 |
53 |
Methylbenzoate |
1.8 |
2.217 |
2.217 |
1.64 |
0.21 |
43 |
Naphthalene |
2.75 |
2.827 |
2.827 |
2.37 |
0.37 |
8 |
1,2,3-Trichlorobenzene |
3.16 |
3.024 |
3.025 |
2.60 |
0.42 |
48 |
Phenanthrene |
4.09 |
3.462 |
3.463 |
3.12 |
0.49 |
28 |
4,4'-DDT solution (100 ng/μl) |
5.63 |
6.075 |
6.080 |
6.24 |
0.80 |
25 |
Test substance |
-- |
6.938 |
6.922 |
7.25 |
0.86 |
1210 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In a raliable study using the HPLC-method according to OECD 121 for estimating the adsorption coefficient Koc on soil and sewage sludge, for the test item a log Koc of >5.0 was determined. The reference substance of highest log Koc used was DDT (log Koc 5.63). The linear regression from the calibration curve gives a solid result with R^2= 0.9869. Thus, by linear extrapolation a log Koc of 6.2 was estimated for the test item.
- Executive summary:
In a reliable study (not performed under GLP: reliability category 2) the sorption behaviour (log Koc) of the test substance on soil and on sewage sludge was estimated using high performance liquid chromatography (HPLC) according to the OECD Guideline for the Testing of Chemicals 121 (2001). 7 compounds with log Koc-values between 1.25 and 5.63 were used for calibration. From the capacity factor k' of 7.25 for the test item and the calibration curve a log Koc of >5.0 was determined.
The reference substance of highest log Koc used was DDT (log Koc 5.63). The linear regression from the calibration curve gives a solid result with R^2= 0.9869. Thus, by linear extrapolation a log Koc of 6.2 was estimated for the test item.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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