Registration Dossier

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report date:
2003

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Ephedrine hydrochloride
EC Number:
200-074-6
EC Name:
Ephedrine hydrochloride
Cas Number:
50-98-6
IUPAC Name:
2-(methylamino)-1-phenylpropan-1-ol hydrochloride
Details on test material:
- Name of test material (as cited in study report): (-)-Ephedrin-Hydrochlorid
- Lot/batch No.: 010722AX10
- Analytical purity: 100 %

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test medium of the highest test concentration, 1000 mg/l (nominal) was prepared. Susequently the lower test concentrations were prepared by a serial dilution.

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
Activated sludge from laboratory wastewater plant treating municipal sewage.
- Initial biomass concentration: 1 g/l

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
30 min

Test conditions

Test temperature:
20 +/- 2 °C
pH:
before correction: 6.7 - 6.8
after correction: 7.2 - 7.4
Nominal and measured concentrations:
Nominal test substance concentrations:
248, 496, and 1000 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer-vessel (250 ml)
- Fill volume: 250 ml
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: 8 ml/vessel 100-fold concentrated OECD medium

Results and discussion

Effect concentrationsopen allclose all
Duration:
30 min
Dose descriptor:
other: EC20
Effect conc.:
ca. 700 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
30 min
Dose descriptor:
other: EC80
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
30 min
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate

Applicant's summary and conclusion