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Ecotoxicological information

Toxicity to microorganisms

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Description of key information

The estimated protozoan, Tetrahymena pyriformis, 48-hr EL50 value for this substance is 4.733 mg/L based on growth inhibition. 

Key value for chemical safety assessment

Additional information

The hydrocarbons, C9, aromatics, estimated protozoan, Tetrahymena pyriformis, 48-hr EL50 and NOELR values for this substance are 21.16 and 4.73 mg/L, respectively, based on growth inhibition

In this methodology, different loadings of the substance are added to the test medium and equilibrium between the water and the hydrocarbons is achieved according to their specific water solubility. The hydrocarbon concentrations at equilibrium will be characteristic of the loading of the substance, and therefore toxicity values from WAF studies are expressed as effect loadings or lethal loadings (EL or LL), not as concentrations. These effect loadings are used to determine environmental classifications. The WAF methodology is widely accepted for the testing of complex hydrocarbon substances and other UVCBs, and it has been incorporated in different guidance documents, including the REACH guidance (see document in Section 13)