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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP Guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1997
Report date:
1997

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Principles of method if other than guideline:
Official Journal of the European Union of the European Union L 133 part C.
(Amtsblatt der EG L133 Teil C)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
4-isopropylaniline
EC Number:
202-797-2
EC Name:
4-isopropylaniline
Cas Number:
99-88-7
Molecular formula:
C9H13N
IUPAC Name:
4-(propan-2-yl)aniline

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: stock solution , Sample weight is transferd directly to the test vessel

Test organisms

Test organisms (species):
activated sludge
Details on inoculum:
- Laboratory culture: laboratory treatment plant with predominantly municipal sewage (South Wupper commune)
- Initial biomass concentration: 400 mg/L

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
30 min

Test conditions

Test temperature:
20 ± 2 °C
pH:
7.7
Nominal and measured concentrations:
Nominal concentrations: 100, 180, 320, 560, 1000 mg/L and abiotic oxygen consumption control with 10000 mg/L
Reference substance (positive control):
yes
Remarks:
3,5 dichlorphenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
30
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
30 min
Dose descriptor:
EC50
Effect conc.:
437 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate

Any other information on results incl. tables

test results

 Test concentration  respiration rate (mg/L*h)  respiration abiotic (mg/L*h)  Inhibition  
 100  27.6  27.6  0.0  
 180  24.0  24.0  9.4  
 320  20.4  20.4  23.0  
 560  8.6  8.6  67.5  
 1000  1.7  1.7  93.6  
 control mean value  26.5      

Applicant's summary and conclusion

Executive summary:

For 4-isopropylaniline the NOEC (30min) is 100 mg/L and the EC50 (30min) is 437 mg/L. The conducted study followed the OECD guideline 209.