Registration Dossier
Registration Dossier
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EC number: - | CAS number: -
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
ErC50 (72 h) = 23.7 µg a.i./L (mean measured, Pseudokirchneriella subcapitata, OECD guideline 201, growth rate)
NOErC (72 h) = 9.55 µg a.i./L (mean measured, Pseudokirchneriella subcapitata, OECD guideline 201, growth rate)
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 23.7 µg/L
- EC10 or NOEC for freshwater algae:
- 9.55 µg/L
Additional information
In the key study, the effects of the effects of Amines, N-C10-C16-alkyltrimethylenedi-, reaction products with chloroacetic acid on
growth of Pseudokirchneriella subcapitata were assessed (according to OECD guideline 201). The algae were exposed for 72 to nominal concentrations of 2.5, 5.0, 10.0, 20.0 and 40.0 µg a.i./L (measured concentrations (HPLC-MS/MS): 1.1, 2.2, 4.57, 9.55 and 21.1 µg a.i./L). Due to the decrease of the test substance of more than 20% over the exposure period, the test results were based on mean measured concentrations (geometric mean). The EC50 value for growth rate was only slightly higher than the highest test concentration of 21.1 µg a.i./L. It is therefore regarded feasible to use the extrapolated value as endpoint. The NOEC values for growth rate, yield and biomass over 72 h were determined at 9.55 µg a.i./L (mean measured concentration).This result is supported by a second toxicity study performed according to OECD 201 with P. subcapitata. Algae were exposed to nominal substance concentrations of 0.0205, 0.0512, 0.128, 0.320, 0.800 mg/L. The test item concentrations were analytically verified by LC-MS/MS based on the signals of the two lead components C-12 PDA and N’C12-Gly.
The measured test item concentrations based on the lead component C-12 PDA in fresh media at the start of the exposure (0 hours) were in the range of 61 to 100% of the nominal concentrations and < LOQ to 70% at the end (72 hours) of the exposure. The measured test item concentrations based on the lead component N’C12-Gly in fresh media at the start of the exposure (0 hours) were in the range of 97 to 113% of the nominal concentrations and < LOQ to 97% in old media at the end (72 hours) of the exposure. Based on the algal growth rate a nominal based EC50 (72 h) of 0.0301 a.i. mg/l was determined. The NOEC-value for inhibition of growth rate after 72 hours was 0.026 mg a.i./L (nominal).
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