Phosphoric acid, mono- and di-C16-18 (even numbered) alkyl esters, salts with 2,2'-iminodiethanol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Remarks:

See the Read Across Justification

Adequacy of study:

key study

Study period:

1992

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Justification for type of information:

See the Read Across justification document

Reason / purpose for cross-reference:

read-across: supporting information

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 2: Semi-static method)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Name: Phosphoric acid, mixed decyl and octyl esters, compds. with diethanolamine (Phosphorsaeure (C8-C10-Alkyl)-ester, DEA-Salz)
CAS No.: 68425-57-0 (68186-45-8)
Physical state: white solid at 20 °C (turbid, non-pourable paste)

Analytical monitoring:

yes

Remarks:

DOC

Details on sampling:

not specified

Vehicle:

no

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Test organism: Danio rerio
Dource: Fa. Westaquarium of 29.September.1992
Mortality < 0,1 % eine Woche vor dem Einsetzen
no pretreatment
Food: Altromin N-1314 / 180992 1148

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

not specified

Test temperature:

Temperature: 23.0 - 23.5 °C

pH:

pH-values: 6.4 - 7.6 measured in all concentration groups.

Dissolved oxygen:

02 -concentration : 84 - 99 % with a WTW Oxi-91

Salinity:

not specified

Conductivity:

not specified

Nominal and measured concentrations:

nominal concentrations based on DOC determination at 1000 mg/l.

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

>= 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (total fraction)

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1 750 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (total fraction)

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

>= 393.1 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat. (total fraction)

Remarks:

DOC measurement

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

688 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat. (total fraction)

Remarks:

DOC measurement

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Results

Analytical Results

The measured DOC content of the test item was 509.1 mg org. C / g test item. The arithmetic mean of the measured DOC of the test solution after 0, 24h, 72h and 96h is 200 mg DOC/l (39 % of test item DOC). The concentrations in the test solution were below 80 % of nominal in the fresh samples.

Table: Determined DOC concentrations

nominal concentrations		measured concentrations after
test item: mg a.i./l	test item: mg DOC/l	0 h (day 0)  [mg DOC/l]	24 h (day 1) [mg DOC/l]	0 h (day 3) [mg DOC/l]	24 h (day 4) [mg DOC/l]
1000	509.1	203.0	202.0	197.0	298,5
10000	5091.0	3452.5	4065.0	n.d.	n.d.

Table: Mortality results of the test

Concentration [ mg a.i./l]	mortality after x hours
	0	6	24	48	72	96
0	0	0	0	0	0	0
1000	0	0	0	0	0	0
3000	0	100	100	100	100	100
10000	100	100	100	100	100	100

Validity criteria fulfilled:

yes

Conclusions:

According to the results of the ISO 7346-II test with zebrafish Danio rerio, the LC0 (96 h) was determined to be equal or greater than 1000 mg/l (nominal) or 393.1 mg/l (arithmetic mean of measured DOC). The LC50 (96 h) was determined to 1750 mg/L (nominal) or 688.1 mg/l (arithmetic mean of measured DOC).

Executive summary:

The objectives of the study were to determine the effects of the test item on the mortality of the Zebrafish Danio rerio under worst-case exposure conditions, to investigate if fish is the most sensitive organism for the test item and to determine the lethal concentration (LC50), and to determine the no observed effect concentration (NOEC), if possible. The principles were the exposure of fish to test solutions and observation of toxicity symptoms after 6, 24, 48, 72 and 96 hours of exposure under semi-static conditions. The study was performed under GLP conditions according to ISO 7346-II. According to the results of the ISO 7346-II test with zebrafish Danio rerio, the LC0 (96 h) was determined to be equal or greater than 1000 mg/L (nominal) or 393.1 (arithmetic mean of measured DOC). The LC50 (96 h) was determined to 1750 mg/L (nominal) or 688.1 (arithmetic mean of measured DOC).

Description of key information

The toxicity of fish was evaluated with the results of a similar substance, tested according to the ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish[Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 2: Semi-static method). See the Read Across justification.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

688.1 mg/L