Reaction mass of Amines, N-tallow alkyltrimethylenedi-, mono(2-ethylhexanoates), Amines, N-tallow alkyltrimethylenedi-, acetates and n-tallow-1,3-diaminopropane ditallate

Toxicity to microorganisms

Description of key information

No toxicity tests with micro-organisms have been performed with "Reaction mass of Amines, N-tallow alkyltrimethylenedi-, (2-ethylhexanoates), Amines, N-tallow alkyltrimethylenedi-, acetates and n-tallow-1,3 -diaminopropane ditallate" (R814M). The test substance is a salt of tallow-1,3 -diaminopropane (CAS no 1219010 -04 -4) and a mixture of talloil fatty acids, 2 -ethylhexoic and acetic acid.

Under aquatic environmental conditions a large extend of the salt is expected to be dissociated and ecotoxicity observed will originate mainly from tallow-1,3 -diaminopropane. No effects (NOEL>=100 mg/L) were observed for the WAF of tall oil fatty acid to algae and daphnids and for 2 -ethyl hexanoic acid the lowest NOEC (21d daphnia) was 25 mg/L (ECHA). Therefore available toxicity to micro-organisms data on alkyl-1,3 -diaminopropanes were included in the dataset. The ecotoxicity of tallow-1,3 -diaminopropane is expected to be reduced when forming a salt with talloil fatty acids or 2 -ethylhexoic acid.

The lowest 3h EC50 for microorganisms as observed for an alkyl-1,3 -diamino propane in an OECD 209 test is 66 mg/L for oleyl-1,3 -diaminopropane (7173 -62 -8).

As a worst-case the 3h EC50 as observed foroleyl-1,3 -diaminopropane of 66 mg/L is used for riks assessment. This result is recalculated to R814M using the tallow-1,3 -diaminopropane content of 52.2% in R814M. This results in a realistic worst-case EC50 for microorganisms of 126.4 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:

126.4 mg/L

Additional information

No toxicity tests with micro-organisms have been performed with "Reaction mass of Amines, N-tallow alkyltrimethylenedi-, (2-ethylhexanoates), Amines, N-tallow alkyltrimethylenedi-, acetates and n-tallow-1,3 -diaminopropane ditallate" (R814M). This substance is thus a salt of tallow-1,3 -diaminopropane (CAS no 1219010 -04 -4) and a mixture of talloil fatty acids, 2 -ethylhexoic and acetic acid.

Under environmental conditions a large extend of the salt is expected to be dissociated and ecotoxicity observed will originate mainly from tallow-1,3 -diaminopropane. No effects (NOEL>=100 mg/L) were observed for the WAF of tall oil fatty acid to algae and daphnids and for 2 -ethyl hexanoic acid the lowest NOEC (21d daphnia) was 25 mg/L (ECHA). Therefore available toxicity to micro-organisms data on alkyl-1,3 -diaminopropanes were included in the dataset. The ecotoxicity of tallow-1,3 -diaminopropane is expected to be reduced when forming a salt with talloil fatty acids or 2 -ethylhexoic acid.

The lowest 3h EC50 for microorganisms as observed for an alkyl-1,3 -diamino propane in an OECD 209 test is 66 mg/L for oleyl-1,3 -diaminopropane (7173 -62 -8). This result is considered identical with the 3h EC50 of 68 mg/L observed for the tallow-1,3 -diaminopropane (1219010 -04 -4). For hydrogenated tallow-1,3 -diaminopropane (133779 -11 -0) a lower toxicity to micro-organisms is observed with an 3h EC50 of 219 mg/Lbecause of the lower anticipated bioavailability.

Finally there is also a toxicity to micro-organisms study available with tallow-1,3 -diaminopropane oleate (CAS no 1307863 -78 -0) which can be considered to be a comparable substance to the talloil fatty acid salt of tallow-1,3 -diaminopropane. This study resulted in a 3h NOEC of 986 mg/L. This result confirms what was previously assumed that the ecotoxicity of tallow-1,3 -diaminopropane is reduced when forming a salt with talloil fatty acids.

As a worst-case the 3h EC50 as observed foroleyl-1,3 -diaminopropane of 66 mg/L is used for riks assessment. This result is recalculated to R814M using the tallow-1,3 -diaminopropane content of 52.2% in R814M. This results in a realistic worst-case EC50 for microorganisms of 126.4 mg/L.