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EC number: 946-360-0 | CAS number: -
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- June 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- (3Z,6Z)-3,7,11-trimethyldodeca-1,3,6,10-tetraene
- Cas Number:
- 28973-99-1
- Molecular formula:
- C15H24
- IUPAC Name:
- (3Z,6Z)-3,7,11-trimethyldodeca-1,3,6,10-tetraene
- Reference substance name:
- (E)-1-methyl-4-(6-methylhepta-2,5-dien-2-yl)cyclohex-1-ene
- Cas Number:
- 25532-79-0
- Molecular formula:
- C15H24
- IUPAC Name:
- (E)-1-methyl-4-(6-methylhepta-2,5-dien-2-yl)cyclohex-1-ene
- Reference substance name:
- (Z)-1-methyl-4-(6-methylhepta-2,5-dien-2-yl)
- Cas Number:
- 29837-07-8
- Molecular formula:
- C15H24
- IUPAC Name:
- (Z)-1-methyl-4-(6-methylhepta-2,5-dien-2-yl)
- Reference substance name:
- (E)-7,11-dimethyl-3-methylenedodeca-1,6,10-triene
- EC Number:
- 242-582-0
- EC Name:
- (E)-7,11-dimethyl-3-methylenedodeca-1,6,10-triene
- Cas Number:
- 18794-84-8
- Molecular formula:
- C15H24
- IUPAC Name:
- 7,11-dimethyl-3-methylenedodeca-1,6,10-triene
- Reference substance name:
- (E)-1-methyl-4-(6-methylhept-5-en-2-ylidene)
- Cas Number:
- 53585-13-0
- Molecular formula:
- C15H24
- IUPAC Name:
- (E)-1-methyl-4-(6-methylhept-5-en-2-ylidene)
- Reference substance name:
- (Z)-1-methyl-4-(6-methylhept-5-en-2-ylidene)
- Cas Number:
- 13062-00-5
- Molecular formula:
- C15H24
- IUPAC Name:
- (Z)-1-methyl-4-(6-methylhept-5-en-2-ylidene)
- Reference substance name:
- 1-methyl-4-(6-methylhepta-1,5-dien-2-yl)
- Cas Number:
- 869843-05-0
- Molecular formula:
- C15H24
- IUPAC Name:
- 1-methyl-4-(6-methylhepta-1,5-dien-2-yl)
- Reference substance name:
- (3Z,6E)-3,7,11-trimethyldodeca-1,3,6,10-tetraene
- Cas Number:
- 26560-14-5
- Molecular formula:
- C15H24
- IUPAC Name:
- (3Z,6E)-3,7,11-trimethyldodeca-1,3,6,10-tetraene
- Reference substance name:
- (Z)-7,11-dimethyl-3-methylenedodeca-1,6,10-triene
- Cas Number:
- 28973-97-9
- Molecular formula:
- C15H24
- IUPAC Name:
- (Z)-7,11-dimethyl-3-methylenedodeca-1,6,10-triene
- Reference substance name:
- 1-methyl-4-(6-methylhept-5-en-2-yl)cyclohexa-1,4-diene
- Cas Number:
- 72345-84-7
- Molecular formula:
- C15H24
- IUPAC Name:
- 1-methyl-4-(6-methylhept-5-en-2-yl)cyclohexa-1,4-diene
- Reference substance name:
- 2,6,10-trimethyldodeca-2,6,9,11-tetraene
- EC Number:
- 207-948-6
- EC Name:
- 2,6,10-trimethyldodeca-2,6,9,11-tetraene
- Cas Number:
- 502-61-4
- Molecular formula:
- C15H24
- IUPAC Name:
- 3,7,11-trimethyldodeca-1,3,6,10-tetraene
- Reference substance name:
- Likely sesquiterpene hydrocarbons
- Cas Number:
- n/a
- Molecular formula:
- C15H24
- IUPAC Name:
- Likely sesquiterpene hydrocarbons
- Test material form:
- liquid
- Details on test material:
- UVCB substance
Constituent 1
Constituent 2
Constituent 3
Constituent 4
Constituent 5
Constituent 6
Constituent 7
Constituent 8
Constituent 9
Constituent 10
Constituent 11
Constituent 12
- Specific details on test material used for the study:
- Batch VE00397335
Purity Chromatogram conform
Expiration date August 18, 2016
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Fresh activated sludge from a biological waste water treatment plant treating predominantly domestic sewage
The sludge is collected in the morning, washed three times in the mineral medium (by centrifuging at 1000 g for 10 minutes, discarding the supermatant and resuspending in mineral medium) and kept aerobic until being used on the same day. - Duration of test (contact time):
- 67 d
Initial test substance concentration
- Initial conc.:
- 30 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- Performance of the test:
Everyday the oxygen consumption of each flask is recorded and correct temperature and stirring are checked.
At the end of the test period (normally 28 days), the pH of each flask is measured again.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
- Test performance:
- Everyday the oxygen consumption of each flask is recorded and correct temperature and stirring are checked.
At the end of the test period (normally 28 days), the pH of each flask is measured again.
% Degradationopen allclose all
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 58
- Sampling time:
- 28 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 65
- Sampling time:
- 42 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 67
- Sampling time:
- 67 d
BOD5 / COD results
- Results with reference substance:
- Degradation of sodium benzoate exceeded 40% after 7 days and 65% after 14 days: the activity of the inoculum was thus verified (validity criterion).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- Bisabolene undergoes 58% biodegradation after 28 days (65% biodegradation after 42 days, 67% biodegradation after 67 days) in the test conditions.
Bisabolene did not inhibit the intrinsic respiration of the inoculum at the test concentration and was therefore considered to be non-toxic to the inoculum at the test concentration.
Therefore, Bisabolene should be regarded as not readily biodegradable according to this test. However, based on these results Bisabolene should be regarded as inherently and ultimately biodegradable. - Executive summary:
The ready biodegradability of Bisabolene was determined by the Manometric Respirometry test (OECD Guideline No. 301F).
Bisabolene undergoes 58% biodegradation after 28 days (65% biodegradation after 42 days, 67% biodegradation after 67 days) in the test conditions.
Bisabolene did not inhibit the intrinsic respiration of the inoculum at the test concentration and was therefore considered to be non-toxic to the inoculum at the test concentration.
Therefore, Bisabolene should be regarded as not readily biodegradable according to this test. However, based on these results Bisabolene should be regarded as inherently and ultimately biodegradable.
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