Reaction product of mixed inorganic base and acid resulting in complex boron, potassium and fluoride constituents in powder form.

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study. Pre dates OECD Guideline

Qualifier:

according to guideline

Guideline:

other: ASTM Standard E 729-80

GLP compliance:

not specified

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnid
- Source: Brood stock
- Age at study initiation (mean and range, SD): < 24h
- Method of breeding: The brood stock was cultured and maintained in aerated (90-105% saturation) 2.91 - liter animal jars. A 2.0-liter open-ended tube with 1.0 mm nylon bolting cloth affixed to the bottom was contained within each jar to facilitate harvesting first instars. Th screened end of the tube was held approximately 1.5 cm off the bottom by two Pyrex triangles placed one on top of the other. Each tube contained 25 adult daphnids. When the tube was lifted, the adults remained on the screen while the first instars passed into the beaker for harvesting. Each brood stock animal jar contained the appropriate amount of food and water made up to a 2.0 liter volume. The brood stock food, water and glass receptacles were changed 3 time per week. The brood stock was kept in an environmental chamber set at 20 +-1°C and a photoperiod of 16h light : 8h dark. Twenty-four hours before testing, mutiparous females were isolated. The neonates produced by these adults were removed and used for testing within 24h.
- Feeding during test : no feeding during test

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

148 +- 7 mg/L as CaCO3

Test temperature:

20.1 - 20.7

pH:

6.7 - 8.1

Dissolved oxygen:

> 60% saturation

Nominal and measured concentrations:

The nominal concentrations were : 54, 91, 151, 252, 420 and 700 mg/l as boron

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 250 ml beakers to which 200 ml of the appropriate amount of test material and water was added.
- Aeration: no aeration during test
- No. of organisms per vessel: 10 neonates


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Lake Huron water was used to culture daphnids and as the test dilution water. This water was carbon-filtered, UV-irradiated and adjusted to a hardness of approximately 170 mg/L as (CaCO3) with CaCl2. The water was then autoclaved at 120°C and 124.1 kPa for 35 min.
- Alkalinity:58 +- 5 mg/L as CaCO3
- Conductivity: 290 +- 21 µmhos/cm


OTHER TEST CONDITIONS
- Photoperiod: 16h light : 8 h dark


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality was recorded after 24 and 48h of exposure

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

133 mg/L

Nominal / measured:

nominal

Conc. based on:

element

Basis for effect:

mortality

Remarks on result:

other: 115-153 mg/L

Details on results:

- Mortality of control: 7%

Reported statistics and error estimates:

The LC50 and 95% confidence intervals were determined. The LC50 values were based on nominal concentrations. Finney's method of probit analysis or the moving angle method was used to calculate the LC50 values.

Duration	Endpoint	Effect conc.	Nominal/Measured	Conc. based on	Basis for effect	Remarks (e.g. 95% CL)
48 h	LC50	133 mg/L	nominal	element	mortality
48 h	LC0	54 mg/L	nominal	element	mortality
48 h	LC100	420 mg/L	nominal	element	mortality

Additional information

Short term effects on aquatic invertebrates ranged from 45 to 1376 mg B/L.

These data support a conclusion that borates should not be classified as dangerous for the environment.