Registration Dossier
Registration Dossier
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EC number: 944-982-7 | CAS number: -
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- January 03 to May 18, 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.3110 (Ready Biodegradability)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- ethyl 2-cyclopentylidene-3-oxobutanoate
- EC Number:
- 812-640-5
- Cas Number:
- 95296-11-0
- Molecular formula:
- C11H16O3
- IUPAC Name:
- ethyl 2-cyclopentylidene-3-oxobutanoate
- Reference substance name:
- ethyl 2-(cyclopent-1-en-1-yl)-3-oxobutanoate
- Molecular formula:
- C11H16O3
- IUPAC Name:
- ethyl 2-(cyclopent-1-en-1-yl)-3-oxobutanoate
Constituent 1
Constituent 2
- Specific details on test material used for the study:
- Aspect : Colourless to pale yellow liquid
Lot No. : SC00018589
Purity : 93.8% (sum of the three peaks)
Expiration date : June 07, 2020
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- Fresh activated sludge from a biological waste water treatment plant treating predominantly domestic sewage (Bois-de-Bay, Satigny, Switzerland) was used.
The sludge is collected in the morning, washed three times in the mineral medium (by centrifuging at 1000 g for 10 minutes, discarding the supernatant and resuspending in mineral medium) and kept aerobic until being used on the same day. - Duration of test (contact time):
- 61 d
Initial test substance concentration
- Initial conc.:
- 30 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradationopen allclose all
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 66
- Sampling time:
- 42 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 75
- Sampling time:
- 61 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 53
- Sampling time:
- 28 d
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- Ethyl Cyclopentylidene Acetoacetate did not significantly inhibit the intrinsic respiration of the inoculum at the test concentration and was therefore considered to be non-toxic to the inoculum at the test concentration.
Ethyl Cyclopentylidene Acetoacetate undergoes 53% biodegradation after 28 days (66% biodegradation after 42 days, 75% biodegradation after 61 days) in the test conditions.
Thus, Ethyl Cyclopentylidene Acetoacetate should be regarded as not readily biodegradable according to this test. However, based on these results Ethyl Cyclopentylidene Acetoacetate should be regarded as inherently and ultimately biodegradable. - Executive summary:
The Ready Biodegradability of Ethyl Cyclopentylidene Acetoacetate was determined by the Manometric Respirometry Test.
Ethyl Cyclopentylidene Acetoacetate did not significantly inhibit the intrinsic respiration of the inoculum at the test concentration and was therefore considered to be non-toxic to the inoculum at the test concentration.
Ethyl Cyclopentylidene Acetoacetate undergoes 53% biodegradation after 28 days (66% biodegradation after 42 days, 75% biodegradation after 61 days) in the test conditions.
Thus, Ethyl Cyclopentylidene Acetoacetate should be regarded as not readily biodegradableaccording to this test. However, based on these results Ethyl Cyclopentylidene Acetoacetate should be regarded as inherently and ultimately biodegradable.
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