Reaction products of 1,5-diaminoanthracene-9,10-dione and 1,8-diaminoanthracene-9,10-dione with bromine

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

February 1987

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: Ciba Geigy Method, please see more details below

Deviations:

not specified

GLP compliance:

no

Analytical monitoring:

no

Details on sampling:

Not applicable

Vehicle:

no

Details on test solutions:

Not applicable

Test organisms (species):

activated sludge

Details on inoculum:

Bacteria used have to be isolated from sewage sludge. This is taken from the aerated compartment of an Husmann apparatus and has to be prepared freshly on a
weekly basis.

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Hardness:

Not available

Test temperature:

25°C

pH:

not available

Dissolved oxygen:

Not available

Salinity:

Not applicable

Conductivity:

Not available

Nominal and measured concentrations:

Tested are 1, 5, 10, 20, 50, 100, 200 and 300 ppm of a test substance. In special cases 1, 3.2, 10, 32, 100, 320 and 1000 ppm are used (as indicated in the short report). 0.2 ml of the dilution is mixed with 15 ml of liquid nutrient agar (tempered to 45 °C) and plated on sterile petri dishes (agar incorporation test). Solvent and growth controls have to be used in parallel. In case of the suspension test 0.1 ml of dilutions are mixed with 4.5 ml of Nutrient broth in a test tube.

Details on test conditions:

Bacterial pre-cultures:
Once per week a pre-culture is prepared taking one inoculation loop of bacteria from an agar plate (BHI-agar) to be cultivated in Nutrient-Broth medium. Each day one inoculation loop from this pre-culture is used to inoculate 5 ml of Nutrient-Broth which is then cultured at 22 °C.

Method:
Inoculation suspension:
In case of tests on bacteria, the over-night culture is used as such.
In case of active sewage sludge testing, sludge is directly used from the Husmann apparatus on day 3 to 5. 100 ml
of underrun is taken on a daily basis and filtered through a Whatman GF/A filter. The filtrate is used as inoculation suspension.

Solvents:
Water, Methylcellusolve (< 2 %) or dimethyl formamide (< 1 %) are used as solvents.

Inoculation:
Agar incorporation test:
Prior to inoculation plates are dried 1-2 hours in an incubator. Plates are inoculated with 1 drop of bacteria suspension. As soon as the liquid has evaporated plates are closed and incubated for 12-24 hours at 25 ± 2 °C in an incubator.

Suspension test:
Test tubes are inoculated with one drop of the bacteria sewage sludge suspension and incubated for 12-24 hours
at 25 ± 2 °C in an incubator.

Reference substance (positive control):

no

Key result

Duration:

21 d

Dose descriptor:

IC50

Effect conc.:

> 400 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: toxicity to activated sewage bacteria

Details on results:

Not available

Results with reference substance (positive control):

Not applicable

Reported statistics and error estimates:

Not available

Validity criteria fulfilled:

not specified

Conclusions:

The bacteria toxicity was determined to be greater than 400 mg/L.

Executive summary:

The toxicity of test substance to activated sludge was determined in a 3-hours respiration inhibition test following procedure similar to the OECD Guideline No. 209.

The bacteria toxicity was determined to be greater than 400 mg/L.

Description of key information

The 3 hr IC50 for a isomeric mixture (FAT 40284) containing the target test substance FAT 36002  was found to be greater than 400 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:

400 mg/L

Additional information

In two studies conducted 1n 1987 the toxicity of test substance to activated sludge was determined in a 3-hours respiration inhibition test following procedure similar to the OECD Guideline No. 209.

The bacteria toxicity data from both study was determined to be greater than 400 mg/L.