Registration Dossier
Registration Dossier
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EC number: 812-508-7 | CAS number: 1093112-19-6
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 05. May 2003 to 08. August 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Cross-reference
- Reason / purpose for cross-reference:
- read-across: supporting information
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- Acute Intrinsic Toxicity of Aluminium sulphate-14-hydrate to Zebrafish (Danio rerio), semi-static exposure
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- certified by Ministerium für Umwelt, Raumordnung und Landwirtschaft des Landes Nordrhein-Westfalen, D-40190 Düsseldorf, Germany
Test material
- Reference substance name:
- Aluminium sulphate
- EC Number:
- 233-135-0
- EC Name:
- Aluminium sulphate
- Cas Number:
- 10043-01-3
- Molecular formula:
- Al.3/2H2O4S
- IUPAC Name:
- aluminium sulphate
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Sampling at the middle depth of the water column at test start, after 24h, 48h, 72h and 96h. At 24h, 48h and 72h the medium was exchanged and both, new and old media were sampled.
A subsample was acidified (pH<2) and designated as "total Aluminium". Another subsample was filtered through a 0.45µm-membrane filter and acidified hereafter. This subsample was designated as "dissolved aluminium".
Test solutions
- Vehicle:
- yes
- Remarks:
- reconstituted water
- Details on test solutions:
- test substance dilauted in reconstituted water, the water was changed after 24h, 48h, 72h and 96h, the pH was adjusted to 7,5 in each vessel
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- healthy fish,length 2cm +/-1cm
laboratory bred (strain obtained from West Aquarium GmbH, D-37431 Lauterberg), reared under study conditions in purified drinking water
fed daily with TetraMin(R) Hauptfutter
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Remarks on exposure duration:
- at 24h, 48h and 72h the medium with test substance was exchanged
- Post exposure observation period:
- no
Test conditions
- Test temperature:
- 22.3 +/- 0.3°C
- pH:
- adjusted to 7.5
- Dissolved oxygen:
- oxygen saturation: ca. 90%
- Salinity:
- freshwater
- Nominal and measured concentrations:
- nominal concentrations: 62.5/125/250/500/1000 mg test substance /L correspondent to 5.63/11.3/22.5/45.0/90.0 mg Al/L
meaured concentrattions (mean): 4.86/10.4/21.6/43.4/85.9 mg Al/L correspondent to 86/92/96/96/95 % of total aluminium - Details on test conditions:
- semi-static (exchanges afte 24h, 48h and 72h), adjusted pH
10 fishes in 10L glass aquaria (loading: 0.2g/L), total of 120 fishes
no feeding during the test as well as 24h before the test
dark/light cycle 14/10
pH-value, oxygen concentration and temperature measured - Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: no mortality observed
- Details on results:
- No mortality observed at the highest concentration (1000 mg aluminium sulphate-14-hydrate) after 96h. The mean measured concentrations (based on Al-detection) differed less than 20% from nominal values.
- Reported statistics and error estimates:
- no mortality observed
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The NOEC for zebrafish in this test is >/= 1000 mg aluminium sulphate-14-hydrate.
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