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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1990-03-01 - 1990-03-20
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Test follows guideline, some deficiences in reporting

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Ethanol, 2,2'-iminobis-, N-coco alkyl derivs., N-oxides
EC Number:
263-180-1
EC Name:
Ethanol, 2,2'-iminobis-, N-coco alkyl derivs., N-oxides
Cas Number:
61791-47-7
Molecular formula:
not applicable
IUPAC Name:
N,N-Bis(2-hydroxyethyl)-C12-18(even numbered) alkyl-1-amine oxides
Details on test material:
Akzo No.; 1-036-013
Product code: 56508
Sample code: 12
Batch No.: 900110
Certificate of analysis: 1990-03-05
Amine oxide content: 30.5%
Free amine: 0.3%
Free peroxide: 0.13%
pH (10+90 water): 7.1
Colour: <2 (Gard.)

Sampling and analysis

Analytical monitoring:
no
Details on sampling:
n.a., measurement of extinction

Test solutions

Vehicle:
no
Details on test solutions:
no

Test organisms

Test organisms (species):
Pseudomonas putida
Details on inoculum:
Pseudomonas putida 50026

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
18 h
Post exposure observation period:
none

Test conditions

Hardness:
no data
Test temperature:
25 deg C
pH:
Potassium hydrogenphosphate KH2PO4/Di Potassium hydrogenphosphate K2HPO4 buffer
Dissolved oxygen:
no data
Salinity:
no
Nominal and measured concentrations:
Nominal test substance (mg/L): 100-500-1000-3000-10000, Control (mg/L): 0
Details on test conditions:
according guideline
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
18 h
Dose descriptor:
EC10
Effect conc.:
702 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
growth inhibition
Details on results:
graphical evaluation
Results with reference substance (positive control):
n.a.
Reported statistics and error estimates:
n.a.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The registration substance has shown a low toxicity to Pseudomonas putida in an 18h growth inhibition test according DIN 38412, part 8 resulting in an EC10 (18h) of 2300 mg/L (nominal, test substance) or 702 mg/L (nominal, active ingredient).