Reaction products of copper phthalocyanine, sulfuric acid, and sodium carbonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

July 31, 1991

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: DIN 38 412

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: "BESTIMMUNG DER WIRKUNG VON WASSERINHALTSSTOFFEN AUF FISCHE - FISCHTEST (L15)"

Deviations:

no

GLP compliance:

no

Details on sampling:

- Concentrations: 100, 215, 464, 1000, 2150 and 4640 mg/l
- Sampling method: The product was added to the test water without any pretreatment

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Golden Orfe
- Source: Fischucht Eggers D-W2354 Hohenwestedt, FRG
- Length at study initiation (length definition, mean, range and SD): 7.3 cm (range: 6.8 - 8.1)
- Weight at study initiation (mean and range, SD): 3.7 g (range: 2.3 - 5.2)
- Method of breeding: ad libitum
- Food type: Growing feed F/B 50
- Corpulence factor: 0.9

ACCLIMATION
- Acclimation period: about 1 month
- Acclimation conditions (same as test or not): same as test
- Mortality during the last 2 weeks of housing: 0.1%
- Mortality during the adaptation period: 0%
- Medical treatment: a few days after arrival prophylactical treatment (twice with 0.05 mg/l malachite green chloride and once with 10 mg/l tetracycline hydrochloride)
- Adaptation to test water and test conditions: 3 days
- Withdrawal of food: 1 day before and during exposure

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

2.5 mmol/l

Test temperature:

20-21 °C

pH:

about 8.0

Dissolved oxygen:

> 60% of maximum saturation

Details on test conditions:

TEST SYSTEM
- Test vessel: All-glass aquarium (30 cm x 22 cm x 24 cm)
- Material, size, headspace, fill volume: flow-throught tank in tap water cleaned by active carbon
- Volume: 10 l
- Aeration: continuous aeration with oil-free air
- Photoperiod: 16h light and 8h darkness
- No. of organisms per vessel: 10


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: the product was added to the test water without any pretreatment; subsequently the fish were placed into the aquaria.
- Ca/mg ratio: 4:1
- Na/k ratio: 10:1
- Acid Capacity: 0.8 mmol/l



TEST CONCENTRATIONS
based on the results of a range finding study (LC 50 after 96 h: between 100 and 1000 mg/l) and a main study not evaluated the concentrations, spaced by a factor of about 2.2, were fixed as follows: 100, 215, 464 and 1000 mg/l.
Since no LC 100 had been achieved the additional concentrations 2150 and 4640 mg/l were added 1 week later.

Reference substance (positive control):

yes

Remarks:

chloroacetamide

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

ca. 792 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

ca. 215 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

ca. 215 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

ca. 2 150 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Observed symptoms: Apathy, Narcotic-like state, Tumbling

Results with reference substance (positive control):

LC50 (48h) = ca. 32 mg/l

Reported statistics and error estimates:

Determination or calculation of the median lethal concentration (LC50) and, if possible the LC5 and the LC95 using the probit analysis[1] after hours (nominal conc): 1, 4, 24, 48, 72 and 96

[1]: "Probit analysis", Finney, d.j., Cambr. Univ. Press, 3rd ed., 1971

Sublethal observations / clinical signs:

Summary results:

LC50 (mg/l; nominal concentrations) after:
1h	> 4600	mg/l
4h	> 2200; < 4600	mg/l
24h	> 1000; < 2200	mg/l
48h	ca. 1000	mg/l
72h	ca. 1000	mg/l
96h	= 792	mg/l	slope factor = 1.49

Validity criteria fulfilled:

not specified

Conclusions:

LC50 (96h) = 792 mg/l

Executive summary:

The substance to be registered has been tested for acute fish toxicity according to DIN 38 412 and has shown a NOEC = 215 mg/l, LC0 = 215 mg/l, LC50 = 792 mg/l and LC100 = 2150 mg/l.

Description of key information

Freshwater: LC50(96h) = 792 mg/l

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

792 mg/L

Additional information

According to the CLP Regulation (EC) n. 1272/2008 ‘acute (short-term) hazard’ means for classification purposes the hazard of a substance or mixture caused by its acute toxicity to an organism during short-term aquatic exposure to that substance or mixture.

The test substance has been tested for acute fish toxicity. The selected key study shows a LC50 = 792 mg/l, this result is confirmed by the other available tests. The Huntsman test (1973) indicates a lower value (302 ppm after 96 h) but no complete information about the sample composition is available.