Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 700-726-4 | CAS number: 1369499-44-4
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- (5Z)-5-(2-methylpropylidene)imidazolidine-2,4-dione
- EC Number:
- 700-726-4
- Cas Number:
- 1369499-44-4
- Molecular formula:
- C7H10N2O2
- IUPAC Name:
- (5Z)-5-(2-methylpropylidene)imidazolidine-2,4-dione
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - content of suspended solids in test batches: 29.6 mg/L
- content of dry matter in the inoculum: 4930 mg/L - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 15.99 mg/L
- Based on:
- DOC
- Details on study design:
- TEST CONDITIONS
- Test temperature: 21.0-21.1°C
- pH: 7.6
TEST SYSTEM
- Culturing apparatus: 2000 mL glass vessels
- Number of culture flasks/concentration: 2 flasks containing test item and inoculum
2 flasks without test item, but with inoculum (=blank batches).
2 flasks containing reference item (Sodium benzoate) and inoculum (=reference batches).
- Measuring equipment: Infrared analyser T 5000 A (Shimadzu)
SAMPLING
- Sampling frequency: 0 and 3 hours, 7, 14, 21, 27 and 28 days
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (DOC removal)
- Value:
- < 0
- Sampling time:
- 28 d
- Details on results:
- Kinetic of test substance (in %):
= 0 after 7 day(s)
< 0 after 14 day(s)
< 0 after 21 day(s)
< 0 after 27 day(s)
< 0 after 28 day(s)
Kinetic of control substance (in %):
> 100 after 14 day(s)
> 100 after 28 day(s)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
