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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
other information
Study period:
2005-11-16 to 2005-12-14 (experimental phase)
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2006
Report date:
2006

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
1,2,3,4-tetrahydro-1-naphthylamine
EC Number:
218-712-7
EC Name:
1,2,3,4-tetrahydro-1-naphthylamine
Cas Number:
2217-40-5
IUPAC Name:
1,2,3,4-tetrahydronaphthalen-1-amine
Details on test material:
- Batch: 7346-05167 Hauptlauf
- Purity: 98.2 area-% (BASF report No. 05L00169)

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge: STP Mannheim, Baden Wuerttemberg, Germany
- Storage conditions: Aeration in the laboratory until use
- Pretreatment: A suitable aliquot of the activated sludge was washed with tap water and sieved by a finely woven mesh with a mesh size about 1 mm and pre-aerated for about 24 hours. The sludge of this suspension was adjusted to a concentration of 6.0 g/L dry weight and then added to the test vessels to obtain a sludge concentration of 30 mg/L dry substance.
- Concentration of sludge: 30 mg/L
Duration of test (contact time):
28 d
Initial test substance concentrationopen allclose all
Initial conc.:
20 mg/L
Based on:
DOC
Initial conc.:
25 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
TEST CONDITIONS
- Composition of medium: According to guideline
- Test temperature: 22 ± 2°C
- Suspended solids concentration: 6 g/L

TEST SYSTEM
- Culturing apparatus: 2L conical flasks, filled up to a volume of 1000 mL
- Number of culture flasks/concentration: 2

SAMPLING
- Sampling frequency: on days 0, 1, 3, 5, 7, 10, 14, 17, 21, 24, 27 and 28

CONTROL AND BLANK SYSTEM
- Inoculum blank: 2
- Abiotic sterile control: 1
- Toxicity control: 1
- Adsorption control: 1

Reference substance
Reference substance:
aniline

Results and discussion

% Degradation
Parameter:
% degradation (DOC removal)
Value:
< 10
Sampling time:
28 d

BOD5 / COD results

Results with reference substance:
Degradation of reference substance after 14 days: 80 - 90%

Any other information on results incl. tables

- Elimination of the test substance by adsorption (% DOC): < 10 after 5 days

- Physico-chemical (abiotic) elimination of the test substance (% DOC): < 10 at the end of exposure

- Biodegradation degree in the inhibition control afier 14 days (% DOC): 40-50

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed