Corsair Clear #12

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

November 19 - 23, 2007

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study has been performed according to OECD and EC guidelines and according to GLP principles.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

Test guideline 3

Qualifier:

equivalent or similar to guideline

Guideline:

ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 1: Static Method)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

- Name of test material (as cited in study report): Corsair Clear #12
- Substance type: Soft waxy beige solid
- Physical state: Solid
- Stability under test conditions: stable
- Storage condition of test material: At room temperature in the dark

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 0 and 100 mg/l
- Sampling method: 40 ml at t = 0 and t = 96 h
- Sample storage conditions before analysis: refrigerator

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Preparation of the test solution started with loading rates of 100 mg/l. The aqueous mixtures were treated with ultrasonic waves for 5 minutes, followed by a 24-hour magnetic stirring period and a 3 hour stabilisation period. The resulting mixtures were slightly hazy with a floating layer,and undissolved test substance was still present on the cover glasses used for the addition of the test substance to the ISO-medium. Therefore, the mixtures were passed over glass wool, after which they were very slightly hazy. Finally, the solutions were combined and used for the test.
- Controls: Test medium without test substance or other additives

Test organisms

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: carp
- Strain: Cyprinus carpio
- Source: Zodiac, proefacc, "De Haar Vissen", Wageningen University and Research Centre, The Netherlands.
- Age at study initiation (mean and range, SD): not indicated
- Length at study initiation (length definition, mean, range and SD): 2.4 ± 0.1 cm
- Weight at study initiation (mean and range, SD): 0.38 ± 0.07 g
- Method of breeding: F1 from a single parent-pair bred in UV-treated water.
- Feeding during test: no feeding

ACCLIMATION
- Acclimation period: At least 12 days after delivery

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

No post exposure observations

Test conditions

Hardness:

180 mg CaCO3 per litre

Test temperature:

21.3 - 22.8°C

pH:

7.4 - 7.8

Dissolved oxygen:

6.3 - 9.6 mg/l

Nominal and measured concentrations:

Loading rate 0 mg/l t=0h : TOC = 0.4425 mg/l
Loading rate 0 mg/l t=96h : TOC = 0.2458 mg/l
Loading rate 100 mg/l t=0h : TOC = 0.5312 mg/l
Loading rate 100 mg/l t=96h: TOC = 0.2229 mg/l
The TOC contents in treated samples were comparable to those in control samples, indicating that the actual test substance concentration was very low. This is not unexpected considering the presence of a floating layer and undissolved test substance in the solution, which were removed by passing the solution over glass wool.

Details on test conditions:

TEST SYSTEM
- Test vessel: 10 litres, all-glass, containing 9 litres of test solution.
- Aeration: Aeration was introduced after 2 days of exposure and continued for 16 hours.
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.30 g fish/litre

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Adjusted ISO medium, formulated using RO-water (tap-water purified by reverse osmosis) with the following composition: CaCl2.2H2O 211.5 mg/l
MgSO4.7H2O 88.8 mg/l
NaHCO3 46.7 mg/l
KCl 4.2 mg/l
- Total organic carbon: 0.44 mg/l

OTHER TEST CONDITIONS
- Adjustment of pH: 7.7 +/- 0.3
- Photoperiod: 16 hours daily

EFFECT PARAMETERS MEASURED: LC50 daily

Reference substance (positive control):

yes

Remarks:

pentachlorophenol

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

loading rate

Basis for effect:

mortality (fish)

Details on results:

No lethal or sublethal effects were observed in the control or the test concentration.

Results with reference substance (positive control):

- Results with reference substance valid: Yes
- Mortality: 0% at 0.10 mg/l; 100% at 0.22 and 0.46 mg/l
- LC50: 96h-LC50 = 0.15 mg/l with a 95% confidence interval between 0.10 and 0.22 mg/l.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions described in this study, Corsair Clear #12 induced no visible effects in carp at a loading rate of 100 mg/l (NOEC).
Due to the very low solubility of Corsair Clear #12 in water, concentration levels that might be toxic for fish could not be reached. Hence the 96h-LC50 was above a loading rate of 100 mg/l.