Reaction mass of 2,2'-[(3,3'-dichloro[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[N-(2,4-dimethylphenyl)-3-oxobutyramide] and 2,2'-[(3,3'-dichloro[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[N-(2-methylphenyl)-3-oxobutyramide] and 2-[[3,3'-dichloro-4'-[[1-[[(2,4-dimethylphenyl)amino]carbonyl]-2-oxopropyl]azo][1,1'-biphenyl]-4-yl]azo]-3-oxo-N-(o-tolyl)butyramide

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

GLP-study according to relevant guidelines with clear and plausible results.

Qualifier:

according to guideline

Guideline:

ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))

Deviations:

no

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Remarks:

The OECD Principles of Good Laboratory Practice

Analytical monitoring:

no

Details on sampling:

not applicable

Vehicle:

no

Details on test solutions:

The test substance was insoluble in water based on information supplied by the sponsor. Analytical confirmation of the actually dissolved concentration in test medium was not possible as no analytical method was available or could be developed with the necessary sensitivity to measure the soluble fraction of the test substance.
This was based on previous work on a similar compound, which showed that the water solubility was less than 10 µg/L (NOTOX Project 289979). The soluble fraction present in saturated solutions prepared during that project were not distinct from the background responses. All possible efforts were taken to improve the detection limit of the analytical method. However, these were not successful.
Preparation of a test solution at maximum saturation of the test substance in test medium started with a nominal concentration of 100 mg/L. This solution was treated with ultrasonic waves and subsequently magnetically stirred for 4 days. The resulting dispersion was then pre-filtered through a paper filter (Schleicher and Schuell 604) to remove the !arger undissolved test substance particles (ca. > 5µm), followed by filtration through a 0.45 µm membrane filter. The final test solution was a clear and colourless solution.
Note that the use of centrifugation to obtain the water-soluble fraction proved not to be successful as a consequence of the behaviour of the test substance in water, i.e. large amounts remained floating on the water surface.

Test organisms (species):

Daphnia magna

Details on test organisms:

- Species: Daphnia magna (Crustacea, Cladocera) (Straus, 1820)
- Validity of batch: Frequent inspection of the cultures with respect to number of young, appearance of young and parental daphnia and global feeding behaviour.
- Characteristics: For the test selection of young daphnia with an age of < 24 hours.
- Start of each batch: With new-born animals, i.e. less than 3 days old, by placing about 250 of them into 10 litres of medium in an all-glass culture vessel.
- Maximum age of the cultures: 4 weeks
- Renewal of the cultures: After 7 days of cultivation half of the medium twice a week.
- Temperature of medium: 18-22°C, constant within ± 1 °C
- Feeding: Daily, a suspension of fresh water algae.
- Medium: M7, as prescribed by Dr. Elendt-Schneider (Elendt, B.-P., 1990: Selenium deficiency in Crustacea. An ultrastructural approach to antennal damage in Daphnia magna Straus. Protoplasma 154, 25-33).

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

not applicable

Hardness:

250 mg/L expressed as CaCO3

Test temperature:

Blank control during the study: 19.6 - 20.4°C

pH:

At t=0h and t=48h
Control: 7.8 - 7.8
Test samples: 7.8 - 7.9

Dissolved oxygen:

At t=0h and t=48h [mg/L]
Control: 7.8 - 8.0
Test samples: 8.2 - 8.5

Salinity:

not applicable

Nominal and measured concentrations:

As the submission substance was insoluble in water (< 10µg/L), analytical verification was impossible due to sensitivity limitations. Therefore, in a limit test the nominal substance concentration of 100 mg/L in test medium was applied after filtration through a 0.45 µm membrane filter.

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 ml, all glass
- static
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
Composition of medium M7:
ISO-medium: the following chemicals (analytical grade) are dissolved in freshly prepared ultra-pure water (tap water purified by reverse osmosis (milli-RO); Millipore Corp., Bedford, Mass., USA) (mg/L):
CaCl2.2H20 293.8
MgSO4.7H20 123.3
NaHCO3 64.8
KCI 5.8
Medium M7: trace elements, macro nutrients and vitamins are added to freshly prepared ISO-medium to reach the following concentrations:
Trace elements (mg/L):
B 0.125
Fe 0.05
Mn 0.025
Li, Rb and Sr 0.0125
Mo 0.0063
Br 0.0025
Cu 0.0016
Zn 0.0063
Co and I 0.0025
Se 0.0010
V 0.0003
Na2EDTA.2H20 2.5
Macro nutrients (mg/L):
Na2SiO3.9H20 10.0
NaNO3 0.27
KH2PO4 0.14
K2HPO4 0.18
Vitamins (µg/L):
Thiamine 75.0
B12 1.0
Biotin 0.75
The hardness: 250 mg/L expressed as CaCO3 and the pH: 8.0 ± 0.2 after aeration.

OTHER TEST CONDITIONS
- Photoperiod: 16h day
- Aeration: No aeration of the test solutions
- Feeding: no
- Introduction of daphnids: directly after preparation of the test solutions



EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilization, 24 and 48 hours

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Remarks:

Immobilization

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Immobility
The immobility data of the limit test are presented in Table 1. No daphnids became immobilised during the test period in the maximum soluble concentration in test medium.
In the blank-control not more than 10% of the daphnids became immobilised, see table 1.
Under the conditions of the present test, the test item induced no effects in Daphnia magna exposed to a filtered solution prepared at a loading of 100 mg/L (NOEC).
In conclusion: Owing to the extremely low solubility of the test item in water, concentration levels toxic for crustaceans could not be reached. Therefore, the 48h-EC50 for Daphnia magna exceeded the maximum solubility of the test item in water.

Results with reference substance (positive control):

48-hour Acute Toxicity Study in Daphnia magna with K2Cr2O7(NOTOX Project 341696).
The study procedures described in this report were based on the ISO International Standard 6341, the EEC directive 92/69, Part C.2. "Acute toxicity for Daphnia" and the OECD guideline No. 202: "Daphnia sp., Acute Immobilisation Test", Adopted April 4, 1984.
The reference test was carried out to check the sensitivity of the test system as used by NOTOX.
Daphnia were exposed for a maximum of 48 hours to K2Cr2O7 concentrations of 0.10, 0.18, 0.32, 0.56, 1.0 and 1.8 mg/L and to a blank control. Ten daphnia were exposed per concentration.
The reference substance, potassium dichromate (K2Cr2O7, ad. 4864.0500, batch no. K28974764) was obtained from Merck, Darmstadt, Germany.
For details see table 2.
The actual responses in this reference test with K2Cr2O7 are within the ranges of the expected responses at the different concentrations. Hence, the sensitivity of this batch of D. magna was in agreement with the historical data collected at NOTOX.
The 24h-EC50 was 1.3 mg/L with 95% fiducial limits of 1.0 to 1.8 mg/L.
The 48h-EC50 was 0.55 mg/L with 95% fiducial limits of 0.48 to 0.69 mg/L.
The test described above was performed under GLP.

Table 1: Acute immobilisation of daphnia after 24 and 48 hours in the limit test.

Concentration Test substance Nominal (mg/L)	Vessel Number	Number Daphnia exposed	Response at 24 h		Response at 48 h
			number	%	number	%
Blank-control	A	10	1	10	1	10
	B	10	0	0	0	0
100*	A	10	0	0	0	0
	B	10	0	0	0	0

* 0.45 µm filtrate.

Table 2: reference substance

Acute immobilization of daphnia alter 24 and 48 hours in the reference test with potassiumdichromate:

Concentration K2Cr2O7 (mg/L)	Number Exposed	% immobile 24h	% immobile 84h	Expected response (%)After 48 hours^1 Minimal                                Maximal
0.00	10	0	0	0	10^2
0.10	10	0	0	0	10
0.18	10	0	0	0	10
0.32	10	0	0	0	30
0.56	10	0	50	0	100
1.0	10	0	100	40	100
1.8	10	100	100	100	100

¹Based on historical data of the previous years (n>60).

²A maximum response of 10% does not invalidate the results of the test.

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of the present test, the test substance induced no effects in Daphnia magna exposed to a filtered solution prepared at a loading of 100 mg/L (NOEC)

Executive summary:

The effects of the submission substance on Daphnia magna straus had been tested according to the ISO International Standard 6341: "Water quality Determination of the inhibition of the mobility of Daphnia magna Straus - Acute toxicity test, Third edition, 1996-04-01 and equivalent to OECD 202 and EU-method C.2. The test fulfilled all the validity criteria and is of reliability category 1.

Because of the insolubility of the submission substance in water (< 10 µg/L), a nominal concentration of 100 mg/L had been used after filtration (0.45 µm membrane filter) and analytical monitoring had not been possible becaus of sensitivity limitations. At the test concentration, no immobilization of daphnids could be observed. The nominal test concentration of 100 mg/L of the submission substance is therefore the NOEC (48h).

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1995-03-01 - 1995-03-03

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Qualifier:

equivalent or similar to guideline

Guideline:

ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))

Principles of method if other than guideline:

not applicable

GLP compliance:

yes

Remarks:

according to OECD codes of GLP, May 1981, Doc C (81)30 (Final)

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
not applicable

Analytical monitoring:

yes

Details on sampling:

In order to check the test concentrations, samples were taken out from the stock solution (1000), 100, 200, 500 mg/L and the test medium after 0, 6 and 24 hours.

Vehicle:

no

Details on test solutions:

A quantity of 0.5 g of the test item was weighed into 500 mL of test medium. This stock solution (1000 mg/L) was used in the toxicity test.

Test organisms (species):

Daphnia magna

Details on test organisms:

A strain of Daphnia magna (Straus), Cladocera, Crustacea, has been cultured at VKI since 1979. Young animals (less than 24 hours old) from this culture were exposed to a dilution series of the test product.

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

not applicable

Hardness:

250 ± 25 mg/L expressed as CaCO3

Test temperature:

20 +- 1°C

pH:

Control: 7.7-7.9
Concentration groups: 7.7-8.1

Dissolved oxygen:

In % saturation:
Control: 89 - 98
Concentration groups: 90 - 97

Salinity:

not applicable

Nominal and measured concentrations:

Concentrations of total carbon were recalculated to the test item considering 33% carbon in the product as reported by the client and that the ISO medium does not contain organic carbon. The results are shown in table 1, section "any other information on materials and methods".
Tested nominal concentrations were [mg/L]:
10; 20; 50; 100; 200; 500 and 1000

Details on test conditions:

The applied test concentrations were based on the preliminary test in which the following concentrations were chosen: 0.01; 0.10; 1.0; 10 and 100 mg/L. As no immobilisation or other effects were seen in this test, the above-mentioned concentrations were used.

According to the method, a 24 h acute toxicity test on the reference substance potassium dichromate (K2Cr2O7) was performed to check the sensitivity of the test animals. The reference substance was tested in the following concentrations: 0.4, 0.7, 1.0, 1.4, 2.0, and 3.0 mg K2Cr2O7/L.

Twenty animals were exposed at each concentration. The number of immobile animals were recorded after 24 and 48 hours. On this basis the effect concentrations (EC) were determined. The pH and dissolved oxygen were measured at 0, 24, and 48 hours.

The test was run in glass containers with a total capacity of 35 mL, each containing 10 mL of test solution.

Freshly produced ISO-medium was used in the tests. The medium was prepared from deionised water, and salts were added to the water according to the standard procedure (ISO International Standard 6341, 1989. "Water quality Determination of the inhibition of the mobility of Daphnia magna Straus (Cladocera, Crustacea)").
The test medium was changed after 24 hours. In order to check the test concentrations, samples were taken out from the stock solution (1000), 100; 200; 500 mg/L and the test medium after 0, 6 and 24 hours and TC (total carbon) measurements were performed to check the actual concentrations in the test vessels.
Five animals less than 24 hours old were transferred to each of 4 test vessels per concentration by use of a nylon net. The control group consisted of 30 animals, 6 vessels with each 5 animals.
The test was run in a climate room at 20 ± 1°C, 16 h light/ 8 h darkness.

Reference substance (positive control):

yes

Remarks:

potassium dichromate (K2Cr2O7)

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Based on the content of the submission substance in the test item (39.6%): > 396 mg/L

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Based on the content of the submission substance in the test item (39.6%): > 396 mg/L

Duration:

24 h

Dose descriptor:

EC10

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Based on the content of the submission substance in the test item (39.6%): > 396 mg/L

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Based on the content of the submission substance in the test item (39.6%): > 396 mg/L

Details on results:

EC10 and EC50 values for the acute toxicity of the test item could not be calculated as no immobility was observed, and EC10 and EC50 are therefore considered to be > 1000 mg/L.
The test item was estimated not to immobilize the test animals at a nominal concentration of 1000 mg/L corresponding to the estimated actual concentration at the beginning of the test (t = 0). The estimated actual concentration (based upon TC measurements) are reduced after 6 and 24 hours.
The number of immobile control animals was 0%.

Results with reference substance (positive control):

The EC10 and EC50 for K2Cr2O7 were calculated by using the standard procedure Probit (SNV, Probit Analysis. Version 2.3. 1992.01.22. Statens Naturvårdsverk (National Swedish Environmental Protection Board. The data section)).
EC10 (24h) = 1.44 mg/L (C.I. 1.14 - 1.58);
EC50 (24h) = 1.78 (C.I. 1.65 - 1.92);

Reported statistics and error estimates:

not applicable

Validity criteria fulfilled:

yes

Conclusions:

The test item was tested for toxicity on Daphnia magna (Straus) in an acute immobilization test according to OECD 202 and compliant to GLP (reliability 1). EC10 and EC50 (both 24 and 48 hours) had been determined to be larger than the highest tested nominal concentration of 1000 mg/L. The test was performed as semistatic (change of the test medium after 24h). The actual concentration was verified by measurement of total carbon and for the two highest test concentration showed to be within 95% of the nominal concentration.
As the test item is an aqueous dispersion of the submission substance with the submission substance accounting for 39.6% of the test item, the results are considered adequate to fulfill the endpoint requirements. The test results based on the content of the submission substance in the test item would be:
EC10 and EC50 (both 24 and 48 hours) > 396 mg/L.

Executive summary:

The test item was tested for toxicity on Daphnia magna (Straus) in an acute immobilization test according to OECD 202 and compliant to GLP (reliability 2). EC10 and EC50 (both 24 and 48 hours) had been determined to be larger than the highest tested nominal concentration of 1000 mg/L. The test was performed as semistatic (change of the test medium after 24h). The actual concentration was verified by measurement of total carbon and for the two highest test concentration showed to be within 95% of the nominal concentration. As the test item is an aqueous dispersion of the submission substance with the submission substance accounting for 39.6% of the test item, the results of the test based on the content of the submission substance would be: EC10 and EC50 (both 24 and 48 hours) > 396 mg/L.

Reference 3

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

GLP-study according to relevant guidelines with clear and plausible results.

Justification for type of information:

see Rationale and Justification for the Analogue Read-Across Approach used for the Registration of Pigment Yellow 174 (Chapter 13)

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))

Deviations:

no

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Remarks:

The OECD Principles of Good Laboratory Practice

Analytical monitoring:

no

Details on sampling:

not applicable

Vehicle:

no

Details on test solutions:

The test substance was insoluble in water based on information supplied by the sponsor. Analytical confirmation of the actually dissolved concentration in test medium was not possible as no analytical method was available or could be developed with the necessary sensitivity to measure the soluble fraction of the test substance.
This was based on previous work on a similar compound, which showed that the water solubility was less than 10 µg/L (NOTOX Project 289979). The soluble fraction present in saturated solutions prepared during that project were not distinct from the background responses. All possible efforts were taken to improve the detection limit of the analytical method. However, these were not successful.
Preparation of a test solution at maximum saturation of the test substance in test medium started with a nominal concentration of 100 mg/L. This solution was treated with ultrasonic waves and subsequently magnetically stirred for 4 days. The resulting dispersion was then pre-filtered through a paper filter (Schleicher and Schuell 604) to remove the !arger undissolved test substance particles (ca. > 5µm), followed by filtration through a 0.45 µm membrane filter. The final test solution was a clear and colourless solution.
Note that the use of centrifugation to obtain the water-soluble fraction proved not to be successful as a consequence of the behaviour of the test substance in water, i.e. large amounts remained floating on the water surface.

Test organisms (species):

Daphnia magna

Details on test organisms:

- Species: Daphnia magna (Crustacea, Cladocera) (Straus, 1820)
- Validity of batch: Frequent inspection of the cultures with respect to number of young, appearance of young and parental daphnia and global feeding behaviour.
- Characteristics: For the test selection of young daphnia with an age of < 24 hours.
- Start of each batch: With new-born animals, i.e. less than 3 days old, by placing about 250 of them into 10 litres of medium in an all-glass culture vessel.
- Maximum age of the cultures: 4 weeks
- Renewal of the cultures: After 7 days of cultivation half of the medium twice a week.
- Temperature of medium: 18-22°C, constant within ± 1 °C
- Feeding: Daily, a suspension of fresh water algae.
- Medium: M7, as prescribed by Dr. Elendt-Schneider (Elendt, B.-P., 1990: Selenium deficiency in Crustacea. An ultrastructural approach to antennal damage in Daphnia magna Straus. Protoplasma 154, 25-33).

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

not applicable

Hardness:

250 mg/L expressed as CaCO3

Test temperature:

Blank control during the study: 19.6 - 20.4°C

pH:

At t=0h and t=48h
Control: 7.8 - 7.8
Test samples: 7.8 - 7.9

Dissolved oxygen:

At t=0h and t=48h [mg/L]
Control: 7.8 - 8.0
Test samples: 8.2 - 8.5

Salinity:

not applicable

Nominal and measured concentrations:

As the submission substance was insoluble in water (< 10µg/L), analytical verification was impossible due to sensitivity limitations. Therefore, in a limit test the nominal substance concentration of 100 mg/L in test medium was applied after filtration through a 0.45 µm membrane filter.

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 ml, all glass
- static
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
Composition of medium M7:
ISO-medium: the following chemicals (analytical grade) are dissolved in freshly prepared ultra-pure water (tap water purified by reverse osmosis (milli-RO); Millipore Corp., Bedford, Mass., USA) (mg/L):
CaCl2.2H20 293.8
MgSO4.7H20 123.3
NaHCO3 64.8
KCI 5.8
Medium M7: trace elements, macro nutrients and vitamins are added to freshly prepared ISO-medium to reach the following concentrations:
Trace elements (mg/L):
B 0.125
Fe 0.05
Mn 0.025
Li, Rb and Sr 0.0125
Mo 0.0063
Br 0.0025
Cu 0.0016
Zn 0.0063
Co and I 0.0025
Se 0.0010
V 0.0003
Na2EDTA.2H20 2.5
Macro nutrients (mg/L):
Na2SiO3.9H20 10.0
NaNO3 0.27
KH2PO4 0.14
K2HPO4 0.18
Vitamins (µg/L):
Thiamine 75.0
B12 1.0
Biotin 0.75
The hardness: 250 mg/L expressed as CaCO3 and the pH: 8.0 ± 0.2 after aeration.

OTHER TEST CONDITIONS
- Photoperiod: 16h day
- Aeration: No aeration of the test solutions
- Feeding: no
- Introduction of daphnids: directly after preparation of the test solutions



EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilization, 24 and 48 hours

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Remarks:

Immobilization

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Immobility
The immobility data of the limit test are presented in Table 1. No daphnids became immobilised during the test period in the maximum soluble concentration in test medium.
In the blank-control not more than 10% of the daphnids became immobilised, see table 1.
Under the conditions of the present test, the test item induced no effects in Daphnia magna exposed to a filtered solution prepared at a loading of 100 mg/L (NOEC).
In conclusion: Owing to the extremely low solubility of the test item in water, concentration levels toxic for crustaceans could not be reached. Therefore, the 48h-EC50 for Daphnia magna exceeded the maximum solubility of the test item in water.

Results with reference substance (positive control):

48-hour Acute Toxicity Study in Daphnia magna with K2Cr2O7(NOTOX Project 341696).
The study procedures described in this report were based on the ISO International Standard 6341, the EEC directive 92/69, Part C.2. "Acute toxicity for Daphnia" and the OECD guideline No. 202: "Daphnia sp., Acute Immobilisation Test", Adopted April 4, 1984.
The reference test was carried out to check the sensitivity of the test system as used by NOTOX.
Daphnia were exposed for a maximum of 48 hours to K2Cr2O7 concentrations of 0.10, 0.18, 0.32, 0.56, 1.0 and 1.8 mg/L and to a blank control. Ten daphnia were exposed per concentration.
The reference substance, potassium dichromate (K2Cr2O7, ad. 4864.0500, batch no. K28974764) was obtained from Merck, Darmstadt, Germany.
For details see table 2.
The actual responses in this reference test with K2Cr2O7 are within the ranges of the expected responses at the different concentrations. Hence, the sensitivity of this batch of D. magna was in agreement with the historical data collected at NOTOX.
The 24h-EC50 was 1.3 mg/L with 95% fiducial limits of 1.0 to 1.8 mg/L.
The 48h-EC50 was 0.55 mg/L with 95% fiducial limits of 0.48 to 0.69 mg/L.
The test described above was performed under GLP.

Table 1: Acute immobilisation of daphnia after 24 and 48 hours in the limit test.

Concentration Test substance Nominal (mg/L)	Vessel Number	Number Daphnia exposed	Response at 24 h		Response at 48 h
			number	%	number	%
Blank-control	A	10	1	10	1	10
	B	10	0	0	0	0
100*	A	10	0	0	0	0
	B	10	0	0	0	0

* 0.45 µm filtrate.

Table 2: reference substance

Acute immobilization of daphnia alter 24 and 48 hours in the reference test with potassiumdichromate:

Concentration K2Cr2O7 (mg/L)	Number Exposed	% immobile 24h	% immobile 84h	Expected response (%)After 48 hours^1 Minimal                                Maximal
0.00	10	0	0	0	10^2
0.10	10	0	0	0	10
0.18	10	0	0	0	10
0.32	10	0	0	0	30
0.56	10	0	50	0	100
1.0	10	0	100	40	100
1.8	10	100	100	100	100

¹Based on historical data of the previous years (n>60).

²A maximum response of 10% does not invalidate the results of the test.

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of the present test, the test substance induced no effects in Daphnia magna exposed to a filtered solution prepared at a loading of 100 mg/L (NOEC)

Executive summary:

The effects of the submission substance on Daphnia magna straus had been tested according to the ISO International Standard 6341: "Water quality Determination of the inhibition of the mobility of Daphnia magna Straus - Acute toxicity test, Third edition, 1996-04-01 and equivalent to OECD 202 and EU-method C.2. The test fulfilled all the validity criteria and is of reliability category 1.

Because of the insolubility of the submission substance in water (< 10 µg/L), a nominal concentration of 100 mg/L had been used after filtration (0.45 µm membrane filter) and analytical monitoring had not been possible becaus of sensitivity limitations. At the test concentration, no immobilization of daphnids could be observed. The nominal test concentration of 100 mg/L of the submission substance is therefore the NOEC (48h).

Reference 4

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

supporting study

Justification for type of information:

see Rationale and Justification for the Analogue Read-Across Approach used for the Registration of Pigment Yellow 174 (Chapter 13)

Reason / purpose for cross-reference:

read-across source

pH:

Control: 7.7-7.9
Concentration groups: 7.7-8.1

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Based on the content of the submission substance in the test item (39.6%): > 396 mg/L

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Based on the content of the submission substance in the test item (39.6%): > 396 mg/L

Duration:

24 h

Dose descriptor:

EC10

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Based on the content of the submission substance in the test item (39.6%): > 396 mg/L

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Based on the content of the submission substance in the test item (39.6%): > 396 mg/L

Details on results:

EC10 and EC50 values for the acute toxicity of the test item could not be calculated as no immobility was observed, and EC10 and EC50 are therefore considered to be > 1000 mg/L.
The test item was estimated not to immobilize the test animals at a nominal concentration of 1000 mg/L corresponding to the estimated actual concentration at the beginning of the test (t = 0). The estimated actual concentration (based upon TC measurements) are reduced after 6 and 24 hours.
The number of immobile control animals was 0%.

Results with reference substance (positive control):

The EC10 and EC50 for K2Cr2O7 were calculated by using the standard procedure Probit (SNV, Probit Analysis. Version 2.3. 1992.01.22. Statens Naturvårdsverk (National Swedish Environmental Protection Board. The data section)).
EC10 (24h) = 1.44 mg/L (C.I. 1.14 - 1.58);
EC50 (24h) = 1.78 (C.I. 1.65 - 1.92);

Reported statistics and error estimates:

not applicable

Validity criteria fulfilled:

yes

Conclusions:

The test item was tested for toxicity on Daphnia magna (Straus) in an acute immobilization test according to OECD 202 and compliant to GLP (reliability 1). EC10 and EC50 (both 24 and 48 hours) had been determined to be larger than the highest tested nominal concentration of 1000 mg/L. The test was performed as semistatic (change of the test medium after 24h). The actual concentration was verified by measurement of total carbon and for the two highest test concentration showed to be within 95% of the nominal concentration.
As the test item is an aqueous dispersion of the submission substance with the submission substance accounting for 39.6% of the test item, the results are considered adequate to fulfill the endpoint requirements. The test results based on the content of the submission substance in the test item would be:
EC10 and EC50 (both 24 and 48 hours) > 396 mg/L.

Executive summary:

The test item was tested for toxicity on Daphnia magna (Straus) in an acute immobilization test according to OECD 202 and compliant to GLP (reliability 2). EC10 and EC50 (both 24 and 48 hours) had been determined to be larger than the highest tested nominal concentration of 1000 mg/L. The test was performed as semistatic (change of the test medium after 24h). The actual concentration was verified by measurement of total carbon and for the two highest test concentration showed to be within 95% of the nominal concentration. As the test item is an aqueous dispersion of the submission substance with the submission substance accounting for 39.6% of the test item, the results of the test based on the content of the submission substance would be: EC10 and EC50 (both 24 and 48 hours) > 396 mg/L.

Description of key information

Under the conditions of the present test, the test substance induced no effects in Daphnia magna exposed to a filtered solution prepared at a loading of 100 mg/I (NOEC)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

mg/L

Additional information

Two studies on the acute toxicity towards invertebrates (equivalent to OECD 202 and according to OECD 202, respectively; reliability category 1) for Pigment Yellow 12 and its structural analogue, Pigment Yellow 13 are available. For both of these pigments consistently no toxicity to Daphnia magna could be found in the tests. Nominal concentrations far above the solubility limit of the pigments had been applied. Therefore, the Diarylide Yellow Pigments are to be regarded as acutely nontoxic to invertebrates.