2-Cyclohexen-1-one, 3,4-dihydroxy-2,6,6-trimethyl-, sodium salt (1:1)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study performed in accordance with OECD Guideline and compliant with GLP criteria.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1184842-87-2

Cas Number:

1184842-87-2

IUPAC Name:

1184842-87-2

Details on test material:

- Test substance: Asta-C9 -Diol-Natrium
- State of aggregation: Fluid
- Homogeneity: Homogerious
- Stability: Not stable against acid solution and heat

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

At the start of the test (after 0 hours) samples from vessels which were not stocked with daphnids were analysed and at the end of the test (after 48 h) samples from stocked and unstocked vessels were analysed.

Test solutions

Details on test solutions:

At the beginning of the test the stock solution of the test substance was freshly prepared. A defined amount of the substance was weighed out and suspended directly in the test medium by stirring (30 min at 20°C). The nominal concentration of the stock solution was 100 mg/L. By dilution of this stock solution with test medium the nominal test concentrations were prepared.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

The clone of Daphnia magna STRAUS used was supplied by Institut National de Recherche Chimique Appliquee, France, in 1978. The daphnids are cultured under standard conditions in the laboratory.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.20 - 3.20 mmol/L

Test temperature:

19-21.8 °C

pH:

7.9-8.1

Dissolved oxygen:

8.3-8.6 mg/L

Nominal and measured concentrations:

- Nominal concentrations: 0, 12.5, 25, 50 and 100 mg/L
Test concentrations of 0, 12.5 and 100 mg/L were analytically verfified at t=0h and t=48h.
- Measured concentrations (t=0h): n.d., 12 and 100 mg/L.
- Measured concentrations (t=48h): n.d., 12 and 100 mg/L

Details on test conditions:

- Test vesseis: Test tubes (glass) with flat bottom; nominal volume 20 mL
- Test medium: according to guideline
- Test volume: 10 mL
- Loading (animals/mL) : 1/2
- Number of animals/vessel: 5
- Total number of animals/conc.:20
- Number of parallels/Test conc.: 4
- Number of parallels/Control: 4
- Age of the animals at the start of the test: 2 - 24 hours
- Age of stock animals: 2 - 4 weeks
- Light: Artificial light, about 5 - 6 µE/(m²·s) in the range of 400 - 700 nm
- Photoperiod: Day:night-rhythm: 16:8 hours
- Test parameter: Swimming ability of the test animals visually, after 0, 3, 6, 24 and 48 h
- Feeding: None

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 20 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Details on results:

No effects were seen at any of the concentrations tested. The test material consists of about 20% aqueous solution. Therefore, and based on the acitive incredient, the EC 50 at 48h is reported at >20mg/L.

Reported statistics and error estimates:

Due to the results of the test a statistical evaluation of the data was unnecessary.