Ethanamine, 2-ethoxy-N-(2-methyl-1-phenylpropylidene)-

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2009-2010

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: OSPAR Commission 2006: Protocols on Methods for the Testing of Chemicals Used in the Offshore Industry (Part B was amended in 2006 (OSPAR summary record 06/23/1, §9.7))

Deviations:

yes

Remarks:

Slightly smaller fish was used(3.55 ± 0.26 cm) than recomended in guideline (4-6 cm). This is considered to be of minor importance.

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

Modified according to OSPAR Commission 2006: Protocols on Methods for the Testing of Chemicals Used in the Offshore Industry

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

(E)-2-Ethoxy-N-(2-methyl-1-phenylpropylidene)ethylamine

EC Number:

604-106-3

Cas Number:

139007-06-0

Molecular formula:

C14H21NO

IUPAC Name:

(E)-2-Ethoxy-N-(2-methyl-1-phenylpropylidene)ethylamine

Test material form:

other: liquid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations:(nominial) 0, 25, 50, 100 and 200 mg/l
- Sampling method: 40 ml taken out in 50 ml bottles of all testkoncentrations
- Sample storage conditions before analysis: Frozen at - 20 C right after sampling

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

other: Scophtalmus maximus

Details on test organisms:

TEST ORGANISM
- Common name: Turbot
- Source: Maximus in Thisted, Denmark
- Age at study initiation (mean and range, SD): Juvenile
- Length at study initiation (length definition, mean, range and SD): 3.55 ± 0.26 cm
- Weight at study initiation (mean and range, SD): 1.06 ± 0.22 g

Study design

Test type:

static

Water media type:

saltwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

16.9 ± 0.5 ºC

pH:

7.9

Dissolved oxygen:

>60 %

Salinity:

32 ‰

Nominal and measured concentrations:

Nominal concentrations: 0(control), 2, 5, 10, 25, 50, 100 and 200 mg/l.
Because the substance breaks down very rapidly in contact with water, one of the breakdown products, IBP (CAS no.611-70-1 ), was measured instead to verify concentrations.

Details on test conditions:

TEST SYSTEM
- Test vessels: 20 l aquariums

- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates):
- Biomass loading rate: approx 1 g per l

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Natural seawater, filtered, collected in the North Sea near Hirtshals by Danmarks Fiskeriundersøgelser 26.10.2009
-

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 (light:dark)

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol, CAS No. : 591-35-5

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

61 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- Mortality: yes
- LC50: 0.6 mg/l
- Other:

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

LC50(96 h) of Ethanamine, 2-ethoxy-N-(2-methyl-1-phenylpropylidene)-, CAS No.139007-06-0, is 61 mg/L for Scophtalmus maximus