Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Currently viewing:

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2-hexyloxyethanol
EC Number:
203-951-1
EC Name:
2-hexyloxyethanol
Cas Number:
112-25-4
Molecular formula:
C8H18O2
IUPAC Name:
2-(hexyloxy)ethanol
Details on test material:
- Name of test material (as cited in study report): N-Hexylglykol
- Analytical purity: 98 %
- Impurities (identity and concentrations): up to 0.2% Diethylenglycole-monobutylether, 0.1% Diethylenglycole, 0.1% Diethylenglycole-monohexylether

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
sewage, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal activated sludge taken from a laboratory wastewater treatment plant fed with municipal sewage
Duration of test (contact time):
8 d
Initial test substance concentration
Initial conc.:
20 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
TEST SYSTEM
- Culturing apparatus:
- Number of culture flasks/concentration: 6

CONTROL AND BLANK SYSTEM
- Inoculum blank: 3
- Abiotic sterile control: 3
- Toxicity control: 3
- reference substance: 3

Concentration of test substance: 32 mg/l = 20 mg/l DOC
Water: 500 ml
Nutrient solution: 6 ml
Inoculum: 0.5 ml
33.9 ml stock solution
Additional water: 427.3 - 493.5 ml
Total volume: 1000 ml
Reference substance
Reference substance:
benzoic acid, sodium salt

Results and discussion

% Degradation
Parameter:
% degradation (DOC removal)
Value:
86
Sampling time:
8 d
Details on results:
Kinetic of test substance (in %):
= 3.3 after 1 day
= 85.4 after 5 days

BOD5 / COD results

Results with reference substance:
PC control: 12 % DOC after 8 days
Reference control: 99 % after 5 days

Applicant's summary and conclusion

Interpretation of results:
readily biodegradable