Propylidynetrimethanol, propoxylated, reaction products with ammonia

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material : AbI. Nr. 85-4113
- Analytical purity: 93.9 g/100 g; w(C6H1 1) = 25.4 g/100 g ; w(C3H60) = 68.5 g/100 g

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: at room temperature

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The reference substance was dissolved in demineralized water. Before adjusting the volume, a solution of internal standard (dissolved in demineralized water) was added. To an aliquot of the samples 0.5 ml of the internal standard were added.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

2.48 mM

Test temperature:

19.2 - 20.2 °C

pH:

7.9 - 9.3

Dissolved oxygen:

8.4 -8.9 mg/l

Nominal and measured concentrations:

Nominal test concentrations (mg/l): 3.13, 6.25, 12.5, 25, 50, 100, control
Analytical test concentrations (mg/l): 93 - 98 % recovery rate of the test substance in nominal concentrations of 100 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: flatt bottom glass tubes (20 ml)
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium according to ISO 10706
- Ca/mg ratio: 4:1
- Conductivity: 550 - 650 µS/cm


OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
- Light intensity: ca. 1 - 8 µE/(m²s) at a wave Iength of 400 - 750 nm


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 24 and 48 h

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

6.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

13 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Cumulated immobility

Nominal conc. (mg/l)	cumulative immobility
	24 h	48 h
0	0	0
3.13	0	0
6.25	0	2
12.5	1	6
25	18	20
50	19	20
100	19	20

Conclusions:

No immobilisation was observed during the 48-hour test period at 0 and 3.13 mg/L. The EC50 (48h) was determined to be 13 mg/L for Daphnia magna.

Description of key information

The test substance was demonstrated to have a EC50 (48h) of 13 mg/L for Daphnia magna. 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

13 mg/L

Additional information

The Klimisch 1 study by BASF AG (2002) performed a short-term toxicity test at 3.13, 6.25, 12.5, 25, 50 and 100 mg/L. Twenty daphnids (4 replicates of 5 animals) were exposed to thes concentration and twenty other daphnids functioned as controls. No immobilisation was observed during the 48-hour test period at 0 and 3.13 mg/L. Immobilisation was observed at 6.25 mg/L (2/20 daphnids), 12.5 mg/L (6/20 daphnids), 25 mg/L (20/20 daphnids), 50 mg/L (20/20 daphnids) and 100 mg/L (20/20 daphnids).