Reaction mass of Di-μ-thio-[{bis(2-ethylhexyl)carbamato-S,S’}oxo molybdenum(V)], Di-μ-thio-[{(2-ethylhexyl)carbamato-S,S’}{(branched ditridecyl)carbamato-S,S’}oxo molybdenum(V)] and Di-μ-thio-[{bis(branched ditridecyl)carbamato-S,S’}oxo molybdenum(V)]

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

10th November to 17 December 1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: study conducted in a GLP compliant laboratory according to accepted regulatory guidelines

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 100, 180, 320, 560 and 1000 mg/L
- Sampling method:Water samples were taken from the control and all surviving test groups at 0,24 (old media), and 96 hours (old media) for quantitative analysis.
- Sample storage conditions before analysis: Duplicate samples and samples at 24 (fresh media), 48 and 72 hours (fresh and old media) were
taken and stored frozen (approximately -20DC) for further analysis if necessary.

Details on test solutions:

Identity and concentration of auxiliary solvent for dispersal: None

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Juvenile rainbow trout
- Strain: N/a
- Source: Reputable commercial supplier
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): 4.2 cm (sd = 0.2)
- Weight at study initiation (mean and range, SD): 0.82 g (sd = 0.10)
- Method of breeding: N/a
- Feeding during test: Not feed during the definitive test
- Food type:
- Amount:
- Frequency:

ACCLIMATION
- Acclimation period: 7days
- Acclimation conditions (same as test or not): Yes
- Type and amount of food: commercial trout pellets
- Feeding frequency:daily which was discontinued 24 hours prior to the start of the definitive study
- Health during acclimation (any mortality observed): ca 1% mortality in 7 days period

QUARANTINE (wild caught)
- Duration:
- Health/mortality:

Test type:

semi-static

Water media type:

freshwater

Hardness:

100 mg CaCO3/L

Test temperature:

14C

pH:

7.6 - 8.1

Dissolved oxygen:

9.3 - 9.8 mg O2/L

Salinity:

N/a

Nominal and measured concentrations:

Analysis of the test solutions at 0, 24 and 96 hours showed the measured test concentrations to be below the limit of quantitation of the analytical method. These results were in-line with pre-study recovery analysis performed on filtered WAFs at a loading rate of 1000 mg/I which also showed measured test concentrations of below the limit of quantitation of the analytical method.

Key result

Duration:

96 h

Dose descriptor:

LL50

Effect conc.:

400 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL

Key result

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

180 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

%Concentration loss over test: 8 ...

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity of the test material to the freshwater fish rainbow trout (Oncorhynchus mykiss) has been investigated and gave a 96-Hour LLso* value of 400 mg/I loading rate filtered WAF with 95% confidence limits of 360 - 450 mg/l loading rate filtered WAF. The No Observed Effect Loading rate
was 180 mg/I loading rate filtered WAF.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

400 mg/L

Additional information

The LC50 value refers to nominal loading level resulting from water accomodated fraction (WAF). Water solubility of the substance is < 0.1 mg/l.